Boudi F H, Lothe R A, Taggart R T
Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, MI 48201.
Hum Genet. 1990 Feb;84(3):293-5. doi: 10.1007/BF00200579.
Human pepsinogen (PGA) exhibits extensive polymorphism that can be detected both at the protein and the DNA level. We describe here two restriction fragment length polymorphisms, EcoRI and BglII, which provide for the detection of three of the most common PGA haplotypes (A, B, and C) in the United States population. The relationship of these polymorphisms to each PGA haplotype was determined by analysis of DNA from individuals exhibiting the corresponding protein phenotypes and by analysis of a series of human x mouse somatic cell hybrids containing the individual chromosome 11 homologous from heterozygous individuals exhibiting the AB and AC protein phenotypes. The use of the BglII polymorphism in combination with previously described EcoRI polymorphism provides a very informative marker of 11q13.
人胃蛋白酶原（PGA）表现出广泛的多态性，可在蛋白质和DNA水平上检测到。我们在此描述两种限制性片段长度多态性，即EcoRI和BglII，它们可用于检测美国人群中三种最常见的PGA单倍型（A、B和C）。通过分析表现出相应蛋白质表型的个体的DNA，以及分析一系列包含来自表现出AB和AC蛋白质表型的杂合个体的11号染色体同源物的人-小鼠体细胞杂种，确定了这些多态性与每种PGA单倍型的关系。将BglII多态性与先前描述的EcoRI多态性结合使用，可为11q13提供一个信息丰富的标记。
