Shishido Hajime, Egashira Yusuke, Okubo Shuichi, Zhang Haining, Hua Ya, Keep Richard F, Xi Guohua
Department of Neurosurgery, University of Michigan, Ann Arbor, MI, USA.
Department of Neurosurgery, University of Michigan, Ann Arbor, MI, USA.
J Neurosci Methods. 2015 Mar 30;243:115-9. doi: 10.1016/j.jneumeth.2015.01.035. Epub 2015 Feb 9.
The endovascular perforation model of subarachnoid hemorrhage (SAH) has a large variation in outcomes. This may reflect differences in the SAH size.
Magnetic resonance imaging (MRI) was performed 24h after endovascular perforation in adult male (n=58) and female (n=58) rats. Rats were divided into five grades according to MRI characteristics: grade 0: no SAH or intraventricular hemorrhage (IVH); grade 1: minimal or thin SAH without IVH; grade 2: minimal or thin SAH with IVH; grade 3: thick SAH without IVH; grade 4: thick SAH with IVH. We investigated whether MRI grading scale reflected severity of SAH (determined post mortem) and neurological score.
There was a strong correlation between MRI grading scale and current SAH grading scale (P<0.01) and neurological score (P<0.01) in male rats. In female rats, there was also a strong correlation between MRI grading scale and SAH grading scale (P<0.01) but not with neurological score (P=0.24).
The current grading system is based on the amount of SAH and needs animal euthanasia to evaluate SAH severity. There is no useful grading system to classify severity of SAH without decapitating animals.
We demonstrated a correlation between the MRI grading scale and the current SAH grading scale in an endovascular perforation rat model. The MRI grading scale allows evaluation of SAH severity without euthanizing animals.
蛛网膜下腔出血（SAH）的血管内穿孔模型在结果上存在很大差异。这可能反映了SAH大小的差异。
在成年雄性（n = 58）和雌性（n = 58）大鼠进行血管内穿孔后24小时进行磁共振成像（MRI）。根据MRI特征将大鼠分为五个等级：0级：无SAH或脑室内出血（IVH）；1级：少量或薄层SAH且无IVH；2级：少量或薄层SAH伴有IVH；3级：厚层SAH且无IVH；4级：厚层SAH伴有IVH。我们研究了MRI分级量表是否反映了SAH的严重程度（死后确定）和神经学评分。
雄性大鼠中，MRI分级量表与当前SAH分级量表（P < 0.01）和神经学评分（P < 0.01）之间存在很强的相关性。在雌性大鼠中，MRI分级量表与SAH分级量表之间也存在很强的相关性（P < 0.01），但与神经学评分无关（P = 0.24）。
当前的分级系统基于SAH的量，并且需要对动物实施安乐死以评估SAH的严重程度。没有一种有用的分级系统可以在不斩首动物的情况下对SAH的严重程度进行分类。
我们在血管内穿孔大鼠模型中证明了MRI分级量表与当前SAH分级量表之间的相关性。MRI分级量表允许在不使动物安乐死的情况下评估SAH的严重程度。
