Correia Rafaela Coelho, Jozala Angela Faustino, Martins Kelly Fernanda, Penna Thereza Christina Vessoni, Duek Eliana Aparecida de Rezende, Rangel-Yagui Carlota de Oliveira, Lopes André Moreni
Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo - FCF/USP, Av. Prof. Lineu Prestes, 580, B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.
World J Microbiol Biotechnol. 2015 Apr;31(4):649-59. doi: 10.1007/s11274-015-1819-0. Epub 2015 Feb 13.
The use of poly(lactic-co-glycolic acid) (PLGA) matrix as a biomolecule carrier has been receiving great attention due to its potential therapeutic application. In this context, we investigated the PLGA matrix capacity to incorporate nisin, an antimicrobial peptide capable of inhibiting the growth of Gram-positive bacteria and bacterial spores germination. Nisin-incorporated PLGA matrices were evaluated based on the inhibitory effect against the nisin-bioindicator Lactobacillus sakei. Additionally, the PLGA-nisin matrix stability over an 8-months period was investigated, as well as the nisin release profile. For the incorporation conditions, we observed that a 5 h incorporation time, at 30 °C, with 250 μg/mL nisin solution in PBS buffer pH 4.5, resulted in the highest inhibitory activity of 2.70 logAU/mL. The PLGA-nisin matrix was found to be relatively stable and showed sustained drug delivery, with continuous release of nisin for 2 weeks. Therefore, PLGA-nisin matrix is could be used as a novel antimicrobial delivery system and an alternative to antibiotics incorporated into PLGA matrices.
聚乳酸-乙醇酸共聚物（PLGA）基质作为生物分子载体因其潜在的治疗应用而备受关注。在此背景下，我们研究了PLGA基质结合乳酸链球菌素的能力，乳酸链球菌素是一种能够抑制革兰氏阳性菌生长和细菌孢子萌发的抗菌肽。基于对乳酸链球菌素生物指示剂清酒乳杆菌的抑制作用，对结合了乳酸链球菌素的PLGA基质进行了评估。此外，还研究了PLGA-乳酸链球菌素基质在8个月期间的稳定性以及乳酸链球菌素的释放曲线。对于结合条件，我们观察到在30℃下，将250μg/mL乳酸链球菌素溶液置于pH 4.5的PBS缓冲液中，结合时间为5小时，可产生最高抑制活性，为2.70 logAU/mL。发现PLGA-乳酸链球菌素基质相对稳定，并显示出持续的药物递送，乳酸链球菌素持续释放2周。因此，PLGA-乳酸链球菌素基质可作为一种新型抗菌递送系统，替代结合到PLGA基质中的抗生素。
