Walter Johannes, Sherwood Peter J, Lin Wei, Segets Doris, Stafford Walter F, Peukert Wolfgang
†Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Institute of Particle Technology (LFG), Cauerstrasse 4, 91058 Erlangen, Bavaria, Germany.
‡Interactive Technology Inc., P.O. Box 2768, Oakland, California 94602, United States.
Anal Chem. 2015 Mar 17;87(6):3396-403. doi: 10.1021/ac504649c. Epub 2015 Mar 3.
Analytical ultracentrifugation (AUC) has proven to be a powerful tool for the study of particle size distributions, particle shapes, and interactions with high accuracy and unrevealed resolution. In this work we show how the analysis of sedimentation velocity data from the AUC equipped with a multiwavelength detector (MWL) can be used to gain an even deeper understanding of colloidal and macromolecular mixtures. New data evaluation routines have been integrated in the software SEDANAL to allow for the handling of MWL data. This opens up a variety of new possibilities because spectroscopic information becomes available for individual components in mixtures at the same time using MWL-AUC. For systems of known optical properties information on the hydrodynamic properties of the individual components in a mixture becomes accessible. For the first time, the determination of individual extinction spectra of components in mixtures is demonstrated via MWL evaluation of sedimentation velocity data. In our paper we first provide the informational background for the data analysis and expose the accessible parameters of our methodology. We further demonstrate the data evaluation by means of simulated data. Finally, we give two examples which are highly relevant in the field of nanotechnology using colored silica and gold nanoparticles of different size and extinction properties.
分析超速离心法（AUC）已被证明是一种用于研究颗粒大小分布、颗粒形状以及相互作用的强大工具，具有高精度和未被揭示的分辨率。在这项工作中，我们展示了如何利用配备多波长检测器（MWL）的AUC沉降速度数据分析，来更深入地理解胶体和大分子混合物。新的数据评估程序已集成到SEDANAL软件中，以处理MWL数据。这开辟了各种新的可能性，因为使用MWL-AUC可以同时获得混合物中各个组分的光谱信息。对于具有已知光学性质的系统，可以获取混合物中各个组分的流体动力学性质信息。首次通过沉降速度数据的MWL评估，展示了混合物中各组分单独消光光谱的测定。在我们的论文中，我们首先提供数据分析的信息背景，并揭示我们方法可获取的参数。我们还通过模拟数据演示了数据评估。最后，我们给出两个在纳米技术领域高度相关的例子，使用不同大小和消光特性的彩色二氧化硅和金纳米颗粒。
