Perocco P, Santucci M A, Gasperi Campani A, Forti G C
Institute for Cancer Research, University of Bologna, Italy.
Teratog Carcinog Mutagen. 1989;9(2):75-81. doi: 10.1002/tcm.1770090203.
The cytotoxic and mutagenic effects of the fungicides mancozeb and thiram were studied using human peripheral blood lymphocytes cultured in vitro with or without an S-9 mix microsomal metabolizing system. The results obtained suggested that the chemicals caused dose-dependent inhibition of thymidine uptake and unscheduled DNA synthesis on both resting and proliferating lymphocytes in the absence of the S-9 mix. In the presence of the S-9 mix, only thiram showed mutagenic activity by eliciting unscheduled DNA synthesis and a significantly higher frequency of sister chromatid exchanges than did controls.
使用在有或没有S - 9混合微粒体代谢系统的情况下体外培养的人外周血淋巴细胞，研究了杀菌剂代森锰锌和福美双的细胞毒性和诱变作用。获得的结果表明，在没有S - 9混合系统的情况下，这些化学物质对静止和增殖淋巴细胞的胸苷摄取和DNA非预定合成均产生剂量依赖性抑制。在有S - 9混合系统的情况下，只有福美双通过引发DNA非预定合成以及显示出比对照组明显更高的姐妹染色单体交换频率而表现出诱变活性。
