Lonergan G, Psachoulia C, Marmaras V, Hondrelis J, Sanida C, Matsoukas J
Department of Chemistry, University of New Brunswick, Fredericton, Canada.
Anal Biochem. 1989 Feb 1;176(2):368-72. doi: 10.1016/0003-2697(89)90325-4.
A general and efficient method for the quantitative isolation of free amino acids from natural sources and their identification as crystalline N-t-butyloxycarbonyl amino acid phenacyl esters is described. The applicability of this method is illustrated in the isolation and characterization of major free amino acids from the Mediterranean fruit fly Ceratitis capitata.
本文描述了一种从天然来源定量分离游离氨基酸并将其鉴定为结晶N-叔丁氧羰基氨基酸苯甲酰酯的通用且高效的方法。该方法在地中海实蝇（Ceratitis capitata）主要游离氨基酸的分离和表征中得到了应用。
