Krasnikova T L, Korichneva I L, Radiukhin V A
Biokhimiia. 1989 Feb;54(2):235-43.
An analysis of human peripheral blood intact mononuclear lymphocyte beta 2-adrenoreceptors showed that the hydrophilic radioligand 3H-CGP-12177 binds at various temperatures only to surface receptors. The density of receptors as determined by the binding of the lipophilic antagonist 125I-CYP at 37 degrees C is 2 times as high as that at 4 degrees C. The affinity of lymphocyte beta 2-adrenoceptors for 1-isoproterenol measured by the labeled ligand displacement at low (4 degrees C) temperatures is by two or three orders of magnitude higher than that at 37 degrees C. The thiol-alkylating agent, N-ethylmaleimide (NEM) causes oppositely directed changes in the density of beta 2-adrenoceptors on intact lymphocytes depending on their original density. NEM increases the affinity of beta 2-adrenoreceptors for the hormone (1.5-12-fold). The state and regulation of human peripheral blood lymphocyte beta 2-adrenoreceptors depend on the hormonal status of patients at the moment of blood sample collection.
对人外周血完整单核淋巴细胞β₂ - 肾上腺素能受体的分析表明，亲水性放射性配体³H - CGP - 12177仅在不同温度下与表面受体结合。通过亲脂性拮抗剂¹²⁵I - CYP在37℃下的结合所测定的受体密度是4℃时的2倍。通过低温（4℃）下标记配体置换法测定的淋巴细胞β₂ - 肾上腺素能受体对1 - 异丙肾上腺素的亲和力比37℃时高两到三个数量级。硫醇烷基化剂N - 乙基马来酰亚胺（NEM）根据完整淋巴细胞上β₂ - 肾上腺素能受体的原始密度导致其密度发生相反方向的变化。NEM增加了β₂ - 肾上腺素能受体对激素的亲和力（1.5至12倍）。人外周血淋巴细胞β₂ - 肾上腺素能受体的状态和调节取决于采血时患者的激素状态。
