激动剂诱导兔视网膜切片和培养物中的糖原分解。

Agonist-induced glycogenolysis in rabbit retinal slices and cultures.

作者信息

Ghazi H, Osborne N N

机构信息

Nuffield Lab. of Opthalmology, Oxford.

出版信息

Br J Pharmacol. 1989 Apr;96(4):895-905. doi: 10.1111/j.1476-5381.1989.tb11900.x.

DOI:10.1111/j.1476-5381.1989.tb11900.x

PMID:2568145

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1854420/

Abstract

The effects of different putative retinal transmitters and/or modulators on glycogenolysis in rabbit retinal slices and in retinal Müller cell cultures were examined. 2. Incubation of rabbit retinal slices or primary retinal cultures (either 3-5 day-old or 25-30 day-old) in a buffer solution containing [3H]-glucose resulted in the accumulation of newly synthesized [3H]-glycogen. 3. Noradrenaline (NA), isoprenaline, vasoactive intestinal peptide (VIP), 5-hydroxytryptamine (5-HT) and 8-hydroxy-dipropylaminetetralin (8-OH-DPAT) stimulated the hydrolysis of this newly formed 3H-polymer. The potency order of maximal stimulations was: VIP greater than NA greater than isoprenaline greater than 5-HT greater than 8-OH-DPAT. 4. The putative retinal transmitters, dopamine, gamma-aminobutyric acid (GABA), glycine and taurine and the muscarinic agonist carbachol (CCh) had no effect on [3H]-glycogen content. 5. The glycogenolytic effects of NA/isoprenaline and 5-HT/8-OH-DPAT appear to be mediated by beta-adrenoceptors and 5-HT1 receptors (possibly 5-HT1A), respectively while the VIP-induced response involved another receptor subtype. 6. Agonists which mediated [3H]-glycogen hydrolysis also stimulated an increase in adenosine 3':5'-cyclic monophosphate (cyclic AMP) formation. Both responses are blocked to a similar extent by the same antagonists and so are probably mediated via the same receptor subtypes. Moreover, dibutyryl cyclic AMP (db cyclic AMP) promoted tritiated glycogen breakdown in the three retinal preparations. 7. Not all receptors linked to cyclic AMP production however promote glycogenolysis. Dopamine and apomorphine stimulated cyclic AMP formation via D1-receptors without influencing glycogenolysis. These receptors are exclusively associated with neurones.

摘要

研究了不同假定的视网膜递质和/或调节剂对兔视网膜切片及视网膜穆勒细胞培养物中糖原分解的影响。2. 将兔视网膜切片或原代视网膜培养物（3 - 5日龄或25 - 30日龄）置于含有[3H] - 葡萄糖的缓冲溶液中孵育，会导致新合成的[3H] - 糖原积累。3. 去甲肾上腺素（NA）、异丙肾上腺素、血管活性肠肽（VIP）、5 - 羟色胺（5 - HT）和8 - 羟基 - 二丙胺四氢萘（8 - OH - DPAT）刺激这种新形成的3H - 聚合物的水解。最大刺激的效力顺序为：VIP＞NA＞异丙肾上腺素＞5 - HT＞8 - OH - DPAT。4. 假定的视网膜递质多巴胺、γ - 氨基丁酸（GABA）、甘氨酸和牛磺酸以及毒蕈碱激动剂卡巴胆碱（CCh）对[3H] - 糖原含量无影响。5. NA/异丙肾上腺素和5 - HT/8 - OH - DPAT的糖原分解作用似乎分别由β - 肾上腺素能受体和5 - HT1受体（可能是5 - HT1A）介导，而VIP诱导的反应涉及另一种受体亚型。6.介导[3H] - 糖原水解的激动剂也刺激了3':5'-环磷酸腺苷（环磷酸腺苷）形成的增加。两种反应都被相同的拮抗剂以相似的程度阻断，因此可能通过相同的受体亚型介导。此外，可以促进三种视网膜制剂中氚化糖原的分解。7. 然而，并非所有与环磷酸腺苷产生相关的受体都能促进糖原分解。多巴胺和阿扑吗啡通过D1受体刺激环磷酸腺苷的形成，而不影响糖原分解。这些受体仅与神经元相关。