Department of Emergency Medicine; Ataturk University Faculty of Medicine, 25240 Erzurum Turkey.
Department of Biochemistry; Agri Ibrahim Cecen University Faculty of Pharmacy, 04100 Agri Turkey.
Environ Toxicol Pharmacol. 2015 Mar;39(2):577-88. doi: 10.1016/j.etap.2015.01.002. Epub 2015 Jan 20.
Contrast medium-induced nephropathy (CIN) remains as a problem with high incidence and mortality rates. The aim of this study is to examine the roles of infliximab (INF) in the glycerol (GLY) and CIN model in rats. The rats were separated into five groups (n=8): Healthy, GLY, GLY+CM, GLY+CM+INF 5mg/kg intraperitoneally (i.p.), and GLY+CM+INF 7 mg/kg (i.p.). Antioxidant levels in the therapy groups were observed to be quite similar to those in the healthy group. In this study, while the kidney TNF-α, IL-1β, TGF-1β and Caspase 3 gene expressions' levels increased in the nephrotoxic groups, these levels were found to have decreased in the treatment groups. Moreover, histopathologic examination showed that hyaline, haemorrhagic casts and necrosis were increased in nephrotoxicity group, whereas they decreased in the therapy group. Furthermore, TNF-α and NF-κB expression were decreased with infliximab administrated groups similar to control group. In conclusion, we suggest that infliximab have protective roles on CIN.
造影剂肾病（CIN）仍然是一种发病率和死亡率都很高的问题。本研究旨在探讨英夫利昔单抗（INF）在甘油（GLY）和 CIN 大鼠模型中的作用。大鼠分为五组（n=8）：健康组、GLY 组、GLY+CM 组、GLY+CM+INF 5mg/kg 腹腔注射（i.p.）组和 GLY+CM+INF 7mg/kg（i.p.）组。治疗组的抗氧化水平与健康组相当。在这项研究中，当肾毒性组的肾 TNF-α、IL-1β、TGF-β1 和 Caspase 3 基因表达水平升高时，治疗组的这些水平则有所下降。此外，组织病理学检查显示，在肾毒性组中透明、出血性铸型和坏死增加，而在治疗组中则减少。此外，用英夫利昔单抗治疗组 TNF-α和 NF-κB 的表达与对照组相似，均有下降。总之，我们认为英夫利昔单抗对 CIN 具有保护作用。
