Suppr超能文献

白细胞介素-4在体外诱导视网膜细胞的胆碱能分化。

IL-4 Induces Cholinergic Differentiation of Retinal Cells In Vitro.

作者信息

Granja Marcelo Gomes, Braga Luis Eduardo Gomes, Carpi-Santos Raul, de Araujo-Martins Leandro, Nunes-Tavares Nilson, Calaza Karin C, Dos Santos Aline Araujo, Giestal-de-Araujo Elizabeth

机构信息

Programa de Neurociências, Departamento de Neurobiologia, Instituto de Biologia, Universidade Federal Fluminense, Outeiro de São João Batista s/n -Campus Valonguinho. Centro - Niterói, Rio de Janeiro, CEP: 24020-140, Brazil.

出版信息

Cell Mol Neurobiol. 2015 Jul;35(5):689-701. doi: 10.1007/s10571-015-0164-z. Epub 2015 Feb 15.

Abstract

Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates several phenomena, among them survival and differentiation of neuronal and glial cells. The aim of this work was to investigate the effect of IL-4 on the cholinergic differentiation of neonatal rat retinal cells in vitro, evaluating its effect on the levels of cholinergic markers (CHT1-high-affinity choline transporter; VAChT-vesicular acetylcholine transporter, ChAT-choline acetyltransferase, AChE-acetylcholinesterase), muscarinic receptors, and on the signaling pathways involved. Lister Hooded rat pups were used in postnatal days 0-2 (P0-P2). Our results show that IL-4 treatment (50 U/mL) for 48 h increases the levels of the cholinergic transporters VAChT and CHT1, the acetylcholinesterase activity, and the number of ChAT-positive cells. It also induces changes in muscarinic receptor levels, leading to a small decrease in M1 levels and a significant increase in M3 and M5 levels after 48 h of treatment. We also showed that IL-4 effect on M3 receptors is dependent on type I IL-4 receptor and on an increase in NFκB phosphorylation. These results indicate that IL-4 stimulates cholinergic differentiation of retinal cells.

摘要

白细胞介素-4(IL-4)是一种多效性细胞因子,可调节多种生理现象,包括神经元和神经胶质细胞的存活与分化。本研究旨在探讨IL-4对新生大鼠视网膜细胞胆碱能分化的影响,评估其对胆碱能标志物(CHT1 - 高亲和力胆碱转运体;VAChT - 囊泡乙酰胆碱转运体、ChAT - 胆碱乙酰转移酶、AChE - 乙酰胆碱酯酶)、毒蕈碱受体水平以及相关信号通路的作用。选用出生后0 - 2天(P0 - P2)的利斯特戴帽大鼠幼崽。我们的结果表明,48小时的IL-4处理(50 U/mL)可提高胆碱能转运体VAChT和CHT1的水平、乙酰胆碱酯酶活性以及ChAT阳性细胞的数量。它还会引起毒蕈碱受体水平的变化,导致处理48小时后M1水平略有下降,M3和M5水平显著升高。我们还表明,IL-4对M3受体的作用依赖于I型IL-4受体以及NFκB磷酸化的增加。这些结果表明,IL-4可刺激视网膜细胞的胆碱能分化。

相似文献

1
IL-4 Induces Cholinergic Differentiation of Retinal Cells In Vitro.
Cell Mol Neurobiol. 2015 Jul;35(5):689-701. doi: 10.1007/s10571-015-0164-z. Epub 2015 Feb 15.
2
The cholinergic system in rat testis is of non-neuronal origin.
Reproduction. 2011 Jul;142(1):157-66. doi: 10.1530/REP-10-0302. Epub 2011 Apr 11.
4
Narcoleptic orexin receptor knockout mice express enhanced cholinergic properties in laterodorsal tegmental neurons.
Eur J Neurosci. 2010 Jul;32(1):130-42. doi: 10.1111/j.1460-9568.2010.07259.x. Epub 2010 Jun 22.
7
Alterations in the non-neuronal acetylcholine synthesis and release machinery in esophageal epithelium.
Life Sci. 2012 Nov 27;91(21-22):1065-9. doi: 10.1016/j.lfs.2012.04.028. Epub 2012 Apr 30.
9
Differential regulation of the high affinity choline transporter and the cholinergic locus by cAMP signaling pathways.
Brain Res. 2007 May 11;1145:1-10. doi: 10.1016/j.brainres.2007.01.119. Epub 2007 Feb 2.

引用本文的文献

1
NLRP3 and autophagy in retinal ganglion cell inflammation in age-related macular degeneration: potential therapeutic implications.
Int J Ophthalmol. 2024 Aug 18;17(8):1531-1544. doi: 10.18240/ijo.2024.08.20. eCollection 2024.
2
Role of Choline in Ocular Diseases.
Int J Mol Sci. 2021 Apr 29;22(9):4733. doi: 10.3390/ijms22094733.

本文引用的文献

3
Neurotransmitters and microglial-mediated neuroinflammation.
Curr Protein Pept Sci. 2013 Feb;14(1):21-32. doi: 10.2174/1389203711314010005.
4
Decreased acetylcholine release delays the consolidation of object recognition memory.
Behav Brain Res. 2013 Feb 1;238:62-8. doi: 10.1016/j.bbr.2012.10.016. Epub 2012 Oct 23.
5
Treatment in vitro of retinal cells with IL-4 increases the survival of retinal ganglion cells: the involvement of BDNF.
Neurochem Res. 2013 Jan;38(1):162-73. doi: 10.1007/s11064-012-0904-0. Epub 2012 Oct 16.
6
Cholinesterases in development: AChE as a firewall to inhibit cell proliferation and support differentiation.
Chem Biol Interact. 2013 Mar 25;203(1):269-76. doi: 10.1016/j.cbi.2012.09.014. Epub 2012 Oct 6.
8
Reconciling neuronally and nonneuronally derived acetylcholine in the regulation of immune function.
Ann N Y Acad Sci. 2012 Jul;1261:7-17. doi: 10.1111/j.1749-6632.2012.06516.x.
9
Overexpression of the vesicular acetylcholine transporter increased acetylcholine release in the hippocampus.
Neuroscience. 2012 Aug 30;218:1-11. doi: 10.1016/j.neuroscience.2012.05.047. Epub 2012 May 26.
10
Substrate-induced internalization of the high-affinity choline transporter.
J Neurosci. 2011 Oct 19;31(42):14989-97. doi: 10.1523/JNEUROSCI.2983-11.2011.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验