珠粒上的磷酸化诱导杂交链式反应：一种用于检测T4多核苷酸激酶活性的超灵敏流式细胞术检测方法。
Phosphorylation-induced hybridization chain reaction on beads: an ultrasensitive flow cytometric assay for the detection of T4 polynucleotide kinase activity.
作者信息
Zhang Yuecheng, Liu Chenghui, Sun Sujuan, Tang Yanli, Li Zhengping
机构信息
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China.
出版信息
Chem Commun (Camb). 2015 Apr 7;51(27):5832-5. doi: 10.1039/c5cc00572h. Epub 2015 Feb 16.
A versatile flow cytometric bead assay (FCBA) has been developed for the ultrasensitive detection of T4 PNK activity by integrating the advantages of the phosphorylation-induced hybridization chain reaction (HCR) for fluorescence signal amplification and flow cytometry for the robust, sensitive and rapid signal readout of the microbeads (MBs).
通过整合磷酸化诱导杂交链式反应（HCR）用于荧光信号放大的优势以及流式细胞术用于微珠（MBs）强大、灵敏且快速的信号读取优势，已开发出一种通用的流式细胞术微珠检测法（FCBA），用于超灵敏检测T4 PNK活性。
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