Fan Wenjiao, Liu Dou, Ren Wei, Liu Chenghui
Key Laboratory of Applied Surface and Colloid Chemistry Ministry of Education, Xi'an, China.
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Xi'an, China.
Front Chem. 2020 Dec 21;8:600317. doi: 10.3389/fchem.2020.600317. eCollection 2020.
Nowadays, the biomolecular assay platforms built-up based on bead counting technologies have emerged to be powerful tools for the sensitive and high-throughput detection of disease biomarkers. In this mini-review, we classified the bead counting technologies into statistical counting platforms and digital counting platforms. The design principles, the readout strategies, as well as the pros and cons of these platforms are introduced in detail. Finally, we point out that the digital bead counting technologies will lead the future trend for the absolute quantification of critical biomarkers, and the integration of new signal amplification approaches and routine optical/clinical instruments may provide new opportunities in building-up easily accessible digital assay platforms.
如今，基于微珠计数技术构建的生物分子检测平台已成为灵敏且高通量检测疾病生物标志物的强大工具。在本综述中，我们将微珠计数技术分为统计计数平台和数字计数平台。详细介绍了这些平台的设计原理、读出策略以及优缺点。最后，我们指出数字微珠计数技术将引领关键生物标志物绝对定量的未来趋势，新信号放大方法与常规光学/临床仪器的整合可能为构建易于使用的数字检测平台提供新机遇。
