Grupo de Espectroscopía Molecular (GEM), Edificio Quifima, Laboratorio de Espectroscopia y Bioespectroscopia, Unidad Asociada CSIC, Parque Científico Uva, Universidad de Valladolid, 47011 Valladolid (Spain) http://www.gem.uva.es.
Angew Chem Int Ed Engl. 2015 Mar 2;54(10):2991-4. doi: 10.1002/anie.201412460. Epub 2015 Feb 12.
Herein we present the first experimental observation of the isolated nucleoside uridine, placed in the gas phase by laser ablation and characterized by Fourier transform (FT) microwave techniques. Free from the bulk effects of their native environments, anti/C2'-endo-g+ conformation has been revealed as the most stable form of uridine. Intramolecular hydrogen bonds involving uracil and ribose moieties have been found to play an important role in the stabilization of the nucleoside.
在此,我们首次观察到了通过激光烧蚀置于气相中的孤立核苷尿嘧啶,并通过傅里叶变换(FT)微波技术对其进行了表征。由于摆脱了其天然环境的本体效应,反/C2'-endo-g+构象被揭示为尿嘧啶最稳定的形式。研究发现,涉及尿嘧啶和核糖部分的分子内氢键在核苷的稳定化中起着重要作用。