Erdmann R, Veenhuis M, Mertens D, Kunau W H
Institute of Physiological Chemistry, Ruhr-University, Bochum, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1989 Jul;86(14):5419-23. doi: 10.1073/pnas.86.14.5419.
Two mutants of Saccharomyces cerevisiae affected in peroxisomal assembly (pas mutants) have been isolated and characterized. Each strain contains a single mutation that results in (i) the inability to grow on oleic acid, (ii) accumulation of peroxisomal matrix enzymes in the cytosol, and (iii) absence of detectable peroxisomes at the ultrastructural level. These lesions (pas1-1 and pas2) are shown to be nonallelic and recessive. Crossing of pas1-1 and pas2 strains resulted in diploid cells that had regained the ability to grow on oleic acid as sole carbon source and to form peroxisomes. These pas mutants may provide useful tools for future studies on the molecular mechanisms involved in peroxisomal assembly.
已分离并鉴定出酿酒酵母中两个过氧化物酶体组装受影响的突变体（pas突变体）。每个菌株都含有一个单一突变，导致：（i）无法在油酸上生长；（ii）过氧化物酶体基质酶在细胞质中积累；（iii）在超微结构水平上未检测到过氧化物酶体。这些损伤（pas1-1和pas2）显示为非等位基因且隐性。pas1-1和pas2菌株杂交产生的二倍体细胞恢复了以油酸作为唯一碳源生长并形成过氧化物酶体的能力。这些pas突变体可能为未来研究过氧化物酶体组装所涉及的分子机制提供有用的工具。
