Zhu Changhua, Yang Na, Ma Xiaoling, Li Guijun, Qian Meng, Ng Denny, Xia Kai, Gan Lijun
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Plant Cell Rep. 2015 Jun;34(6):1025-36. doi: 10.1007/s00299-015-1762-4. Epub 2015 Feb 17.
Our results show that methyl jasmonate induces plasma membrane H (+) -ATPase activity and subsequently influences the apoplastic pH of trichoblasts to maintain a cell wall pH environment appropriate for root hair development. Root hairs, which arise from root epidermal cells, are tubular structures that increase the efficiency of water absorption and nutrient uptake. Plant hormones are critical regulators of root hair development. In this study, we investigated the regulatory role of the plasma membrane (PM) H(+)-ATPase in methyl jasmonate (MeJA)-induced root hair formation. We found that MeJA had a pronounced effect on the promotion of root hair formation in lettuce seedlings, but that this effect was blocked by the PM H(+)-ATPase inhibitor vanadate. Furthermore, MeJA treatment increased PM H(+)-ATPase activity in parallel with H(+) efflux from the root tips of lettuce seedlings and rhizosphere acidification. Our results also showed that MeJA-induced root hair formation was accompanied by hydrogen peroxide accumulation. The apoplastic acidification acted in concert with reactive oxygen species to modulate root hair formation. Our results suggest that the effect of MeJA on root hair formation is mediated by modulation of PM H(+)-ATPase activity.
我们的结果表明，茉莉酸甲酯可诱导质膜H(+) -ATP酶活性，随后影响根毛细胞的质外体pH值，以维持适合根毛发育的细胞壁pH环境。根毛由根表皮细胞产生，是管状结构，可提高水分吸收和养分摄取的效率。植物激素是根毛发育的关键调节因子。在本研究中，我们研究了质膜（PM）H(+)-ATP酶在茉莉酸甲酯（MeJA）诱导的根毛形成中的调节作用。我们发现，MeJA对生菜幼苗根毛形成有显著的促进作用，但这种作用被质膜H(+)-ATP酶抑制剂钒酸盐所阻断。此外，MeJA处理增加了质膜H(+)-ATP酶活性，同时伴随着生菜幼苗根尖H(+)外流和根际酸化。我们的结果还表明，MeJA诱导的根毛形成伴随着过氧化氢的积累。质外体酸化与活性氧协同作用来调节根毛形成。我们的结果表明，MeJA对根毛形成作用是通过调节质膜H(+)-ATP酶活性介导的。
