Graduate Center for Materials Research and Center for Biomedical Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA.
Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
Mater Sci Eng C Mater Biol Appl. 2015 Apr;49:632-639. doi: 10.1016/j.msec.2015.01.060. Epub 2015 Jan 16.
Much work has focused on developing synthetic materials that have tailored degradation profiles and physical properties that may prove useful in developing biomaterials for tissue engineering applications. In the present study, three different composite sheets consisting of biodegradable poly-ε-caprolactone (PCL) and varying types of bioactive glass were investigated. The three composites were composed of 50wt.% PCL and (1) 50wt.% 13-93 B3 borate glass particles, (2) 50wt.% 45S5 silicate glass particles, or (3) a blend of 25wt.% 13-93 B3 and 25wt.% 45S5 glass particles. Degradation profiles determined for each composite showed the composite that contained only 13-93 B3 borate glass had a higher degradation rate compared to the composite containing only 45S5 silicate glass. Uniaxial tensile tests were performed on the composites to determine the effect of adding glass to the polymer on mechanical properties. The peak stress of all of the composites was lower than that of PCL alone, but 100% PCL had a higher stiffness when pre-reacted in cell media for 6weeks, whereas composite sheets did not. Finally, to determine whether the composite sheets would maintain neuronal growth, dorsal root ganglia isolated from embryonic chicks were cultured on composite sheets, and neurite outgrowth was measured. The bioactive glass particles added to the composites showed no negative effects on neurite extension, and neurite extension increased on PCL:45S5 PCL:13-93 B3 when pre-reacted in media for 24h. This work shows that composite sheets of PCL and bioactive glass particles provide a flexible biomaterial for neural tissue engineering applications.
许多工作都集中在开发具有定制降解曲线和物理性能的合成材料上，这些材料可能有助于开发用于组织工程应用的生物材料。在本研究中，研究了三种不同的由可生物降解的聚己内酯（PCL）和不同类型的生物活性玻璃组成的复合片。这三种复合材料由 50wt.%的 PCL 和（1）50wt.%的 13-93 B3 硼酸盐玻璃颗粒，（2）50wt.%的 45S5 硅酸盐玻璃颗粒，或（3）25wt.%的 13-93 B3 和 25wt.%的 45S5 玻璃颗粒的混合物组成。每种复合材料的降解曲线表明，仅含有 13-93 B3 硼酸盐玻璃的复合材料的降解速率比仅含有 45S5 硅酸盐玻璃的复合材料更高。对复合材料进行了单轴拉伸试验，以确定向聚合物中添加玻璃对机械性能的影响。所有复合材料的峰值应力都低于单独的 PCL，但在细胞培养基中预反应 6 周后，100%的 PCL 具有更高的刚度，而复合片则没有。最后，为了确定复合片是否能维持神经元的生长，从胚胎鸡的背根神经节分离出来，在复合片上培养，并测量神经突的生长。添加到复合材料中的生物活性玻璃颗粒对神经突的延伸没有负面影响，并且在培养基中预反应 24 小时后，PCL:45S5 和 PCL:13-93 B3 的神经突延伸增加。这项工作表明，PCL 和生物活性玻璃颗粒的复合片为神经组织工程应用提供了一种灵活的生物材料。
