[CD71在血液系统恶性肿瘤细胞增殖中的表达及其与Ki-67的相关性]

Wei Yuan-Yu, Zhang Xiao-Zhan, Zhang Fan, Li Zhao-Bo, Wang Ning-Ning, Liu Shuai, Yue Bao-Hong

Department of Laboratory Medicine, The First Affiliated Hospital of Zhengzhou University, Faculty of Laboratorial Medicine of Zhengzhou Uniersity, Open Laboratory, Henan Province Key Subject of Clinical Medicine, Zhengzhou 450052, Henan Province, China.

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2015 Feb;23(1):234-40. doi: 10.7534/j.issn.1009-2137.2015.01.044.

OBJECTIVE

This study was to explore the expression of CD71, as a proliferation indicator, on cell proliferaration in hematologic malignancy and its correlation with Ki-67, so as to assess the feasibility of CD71 instead of Ki-67 for assaying cell proliferation by flow cytometry (FCM).

METHODS

(1) Compared with mature B lymphoctyes during stationary phase in peripheral blood from healthy people, the cell cycle and the expression of CD71 and Ki-67 of cell lines from patients with leukemia and lymphoma were examined, the correlation among CD71, S-phase cell fraction (SPF) and Ki-67 were analyzed; (2) Compared with mature B lymphoctyes in bone marrow from non-hematologic disease patients, the expression and correlation of CD71 and Ki-67 of all kinds of leukemic cells and myeloma cells from bone marrow were analyzed by using Ki-67/CD71/CD45/CD123, Ki-67/CD71/CD45/CD20 or Ki-67/CD71/CD45/CD138.

RESULTS

(1) in respect to the expression rate of CD71 on tumor cell lines, the expression rate of CD71 on HL-60 cells was (99.77 ± 0.064)%, the expression rate of CD71 on NB4 cells was (99.23 ± 0.12)%, the expression rate on THP-1 cells was (98.90 ± 0.30)% and the expression rate on K562 cells was (97.03 ± 0.15)% in myelogenous leukemia cell lines, the expression rate of CD71 on Raji cells was (99.35 ± 0.21)% and the expression rate on Mino cell was (96.95 ± 0.42)% in lymphoma cell lines, which were also obviously higher than that on cells of the control group (P < 0.05); (2) in respect to the expression rate of CD71 on tumor cells in bone marrow, the expression rate of CD71 on poorly differentiated AML(M1 and M2) cells was (51.50 ± 19.31)%, the expression rate of CD71 on acute promyelocytic leukemia (AML-M3) cells was (35.71 ± 14.02) %, the expression rate of CD71 on acute monocytic leukemia (AML-M5) cells was (30.54 ± 14.38)%, the expression rate of CD71 on acute T lymphoblastic leukemia cells was (68.40 ± 20.83)%, the expression rate of CD71 on acute B lymphoblastic leukemia was (39.67 ± 18.27)%, the expression rate of CD71 on multiple myeloma (MM) cells was (55.49 ± 18.15%), the expression rate of CD71 on chronic lymphocytic leukemia(CLL) was (1.32 ± 0.33%), which were also higher than that on cells in the control group(P < 0.05) except for CLL cells (P > 0.05); (3) CD71 had a positive linear corrlation with SPF in cell lines (r = 0.914, P < 0.05), and also had a positive linear corrlation with Ki-67 in cell lines and carcinoma cells from bone marrow (r = 0.894,r = 0.904, P < 0.05).

CONCLUSION

The CD71 can take the place of Ki-67 as an indicator of cell proliferation activity of hematologic malignancies and the determination CD71 by FCM is simpler and better than that of Ki-67 in respest of methodology.

目的

本研究旨在探讨增殖指标CD71在血液系统恶性肿瘤细胞增殖中的表达情况及其与Ki-67的相关性，以评估采用流式细胞术（FCM）检测时CD71替代Ki-67检测细胞增殖的可行性。

方法

（1）检测白血病和淋巴瘤患者细胞系的细胞周期以及CD71和Ki-67的表达，并与健康人外周血静止期成熟B淋巴细胞进行比较，分析CD71、S期细胞分数（SPF）和Ki-67之间的相关性；（2）采用Ki-67/CD71/CD45/CD123、Ki-67/CD71/CD45/CD20或Ki-67/CD71/CD45/CD138分析骨髓中各类白血病细胞和骨髓瘤细胞CD71和Ki-67的表达及相关性，并与非血液系统疾病患者骨髓中的成熟B淋巴细胞进行比较。

结果

（1）肿瘤细胞系中，髓系白血病细胞系HL-60细胞CD71表达率为（99.77±0.064）%，NB4细胞为（99.23±0.12）%，THP-1细胞为（98.90±0.30）%，K562细胞为（97.03±0.15）%；淋巴瘤细胞系Raji细胞CD71表达率为（99.35±0.21）%，Mino细胞为（96.95±0.42）%，均显著高于对照组细胞（P<0.05）；（2）骨髓肿瘤细胞中，低分化急性髓系白血病（M1和M2）细胞CD71表达率为（51.50±19.31）%，急性早幼粒细胞白血病（AML-M3）细胞为（35.71±14.02）%，急性单核细胞白血病（AML-M5）细胞为（30.54±14.38）%，急性T淋巴细胞白血病细胞为（68.40±20.83）%，急性B淋巴细胞白血病为（39.67±18.27）%，多发性骨髓瘤（MM）细胞为（55.49±18.15）%，慢性淋巴细胞白血病（CLL）细胞为（1.32±0.33）%，除CLL细胞外（P>0.05），其余均高于对照组细胞（P<0.05）；（3）细胞系中CD71与SPF呈正线性相关（r=0.914，P<0.05），在细胞系及骨髓癌细胞中CD71与Ki-67也呈正线性相关（r=0.894，r=0.904，P<0.05）。