Acharya Seema, Kala Pooja Sharma
Department of Pathology, Sri Guru Ram Rai Institute of Medical and Health Sciences, Dehradun, Uttarakhand, India.
Indian J Pathol Microbiol. 2019 Jul-Sep;62(3):418-422. doi: 10.4103/IJPM.IJPM_604_18.
CD71 or Transferrin receptor is expressed on the surface of erythroid lineage cells. CD71 expression has been found to be significantly increased in rapidly proliferating cells.
This cross-sectional study included 37 bone marrow samples of acute leukemia cases diagnosed between October 2016 to April 2018. The samples were analysed on BD FACS Canto II. We evaluated the expression of CD71 on leukemic blasts and compared median fluorescent intensities (MFI) of blasts in different types of acute leukemias.
The 37 cases comprised of 21 Acute Myeloid Leukemia (AML), 13 B-Acute Lymphoblastic Leukemia (B-ALL), 2 T- Acute Lymphoblastic Leukemia (T-ALL) and 1 mixed phenotypic acute leukemia (MPAL), T/Myeloid. CD 71 expression was noted in 70.3% (n= 26/37) of acute leukemia cases. CD71 expression was most commonly observed in AML (n= 15/21;71.4%), followed by B-ALL (n= 9/13;69.2%) and T-ALL (n= 1/2;50%). Single case of MPAL revealed blasts positive for CD71. MFI of leukemic blasts of single CD71 positive T-ALL was found to be highest, followed by AML, MPAL (T/Myeloid) and least in B ALL. Of the AML cases, the blasts of AML-M6, acute promyelocytic leukemia and AML-M1 showed higher CD71 expression in terms of MFI.
Surface CD71 expression is not only found in erythroid lineage cells, but also in proliferating cells. CD71 MFI is highest in T lymphoblasts followed by leukemic erythroblasts, myeloblasts and least in B lymphoblasts.
CD71 或转铁蛋白受体在红系谱系细胞表面表达。已发现 CD71 在快速增殖细胞中表达显著增加。
这项横断面研究纳入了 2016 年 10 月至 2018 年 4 月期间诊断的 37 例急性白血病患者的骨髓样本。样本在 BD FACS Canto II 上进行分析。我们评估了白血病原始细胞上 CD71 的表达，并比较了不同类型急性白血病中原始细胞的中位荧光强度（MFI）。
37 例患者包括 21 例急性髓系白血病（AML）、13 例 B 淋巴细胞白血病（B-ALL）、2 例 T 淋巴细胞白血病（T-ALL）和 1 例混合表型急性白血病（MPAL），T/髓系。70.3%（n = 26/37）的急性白血病患者存在 CD71 表达。CD71 表达最常见于 AML（n = 15/21；71.4%），其次是 B-ALL（n = 9/13；69.2%）和 T-ALL（n = 1/2；50%）。1 例 MPAL 患者的原始细胞 CD71 呈阳性。发现单一 CD71 阳性 T-ALL 的白血病原始细胞 MFI 最高，其次是 AML、MPAL（T/髓系），B-ALL 最低。在 AML 病例中，AML-M6、急性早幼粒细胞白血病和 AML-M1 的原始细胞在 MFI 方面显示出较高的 CD71 表达。
表面 CD71 表达不仅见于红系谱系细胞，也见于增殖细胞。CD71 MFI 在 T 淋巴母细胞中最高，其次是白血病有核红细胞、髓母细胞，在 B 淋巴母细胞中最低。
