Bälter Magnus, Hammarson Martin, Remón Patricia, Li Shiming, Gale Nittaya, Brown Tom, Andréasson Joakim
Department of Chemical and Biological Engineering, Physical Chemistry, Chalmers University of Technology , 412 96 Göteborg, Sweden.
J Am Chem Soc. 2015 Feb 25;137(7):2444-7. doi: 10.1021/ja512416n. Epub 2015 Feb 17.
We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.
我们表明，共价连接到DNA支架上的太平洋蓝（PB）和Alexa488（A488）之间的荧光共振能量转移（FRET）可以通过螺吡喃衍生物的光致变色切换来可逆控制。当螺吡喃处于闭环螺环异构体形式时，PB和A488之间可自由发生FRET。紫外线诱导的异构化形成开环部花青形式，通过有效淬灭PB激发态来关闭FRET过程。通过暴露于可见光使该过程逆转，触发异构化回到螺环异构体。
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