Borst Christoffer, Xia Shengqiang, Bistrup Claus, Tepel Martin
Department of Nephrology, Odense University Hospital, Odense, Denmark; University of Southern Denmark, Institute of Molecular Medicine, Cardiovascular and Renal Research, Institute of Clinical Research, Odense, Denmark.
Department of Nephrology, Odense University Hospital, Odense, Denmark.
PLoS One. 2015 Feb 17;10(2):e0117315. doi: 10.1371/journal.pone.0117315. eCollection 2015.
Interleukin-8 (IL-8) has been associated with ischemia reperfusion injury after renal allograft transplantation. Impaired allograft function may cause major impact on patient morbidity and health care costs. We investigated whether transcript levels in mononuclear cells including IL-8 on the first postoperative day may be involved in immediate allograft dysfunction as defined by reduced relative change in plasma creatinine at the first postoperative day.
We performed a single center, prospective-cohort study of 113 patients receiving kidney transplants. Peripheral blood mononuclear cells were harvested within 24 hours after transplantation. Transcripts were measured using quantitative RT-PCR.
Transcript levels of IL-8 and S100A8 were significantly lower in patients with relative change in plasma creatinine less than 10% at the first postoperative day. Receiver-operator characteristic curves showed that IL-8 predicted the relative change in plasma creatinine less than 10% (area under curve (AUC), 0.80; P = 0.0007). Multivariate analyses showed that lower IL-8 transcripts, longer time on dialysis, higher recipient body mass index and deceased donor type were associated with relative change in plasma creatinine at the first postoperative day less than 10%.
Reduced levels of IL-8 transcripts in peripheral mononuclear cells predict immediate graft dysfunction and delayed graft function.
白细胞介素-8（IL-8）与肾移植术后缺血再灌注损伤有关。移植肾功能受损可能对患者发病率和医疗费用产生重大影响。我们研究了术后第一天包括IL-8在内的单核细胞转录水平是否与术后第一天血浆肌酐相对变化降低所定义的即刻移植肾功能障碍有关。
我们对113例接受肾移植的患者进行了一项单中心前瞻性队列研究。在移植后24小时内采集外周血单核细胞。使用定量逆转录聚合酶链反应测量转录本。
术后第一天血浆肌酐相对变化小于10%的患者中，IL-8和S100A8的转录水平显著降低。受试者工作特征曲线显示，IL-8可预测血浆肌酐相对变化小于10%（曲线下面积（AUC），0.80；P = 0.0007）。多变量分析显示，较低的IL-8转录本水平、较长的透析时间、较高的受者体重指数和已故供体类型与术后第一天血浆肌酐相对变化小于10%有关。
外周单核细胞中IL-8转录本水平降低预示着即刻移植肾功能障碍和移植肾功能延迟。
