Ciardiello F, Hynes N, Kim N, Valverius E M, Lippman M E, Salomon D S
Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, MD 20892.
FEBS Lett. 1989 Jul 3;250(2):474-8. doi: 10.1016/0014-5793(89)80779-3.
An enhanced expression of transforming growth factor-alpha (TGF alpha) was demonstrated in two clones of NOG-8 mouse mammary epithelial cells, NOG-8 SR1 and NOG-8 SR2, that have been transformed by a v-Ha-ras oncogene. The amount of TGF alpha production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level of p21ras expression in these clones, which directly correlated with their cloning efficiency in soft agar. There was also a decrease in the number of epidermal growth factor (EGF) receptors on the NOG-8 SR1 and NOG-8 SR2 cells that is proportional to the amount of TGF alpha secreted. These effects were specific for ras because neu-transformed NOG-8 cells grew in soft agar at a comparable level to NOG-8 SR2 cells yet did not show any increase in TGF alpha production or change in EGF receptor expression.
在通过v-Ha-ras癌基因转化的NOG-8小鼠乳腺上皮细胞的两个克隆NOG-8 SR1和NOG-8 SR2中，证实了转化生长因子-α（TGFα）的表达增强。NOG-8 SR1和NOG-8 SR2细胞中TGFα的产生量取决于这些克隆中p21ras的表达水平，这与它们在软琼脂中的克隆效率直接相关。NOG-8 SR1和NOG-8 SR2细胞上的表皮生长因子（EGF）受体数量也有所减少，其减少程度与分泌的TGFα量成比例。这些效应是ras特异性的，因为neu转化的NOG-8细胞在软琼脂中的生长水平与NOG-8 SR2细胞相当，但TGFα产生没有任何增加，EGF受体表达也没有变化。
