Schubert M L, Hightower J
Department of Medicine, Medical College of Virginia, Richmond.
Gastroenterology. 1989 Sep;97(3):561-7. doi: 10.1016/0016-5085(89)90625-2.
The effect and mode of action of bombesin on gastric acid secretion was examined in the isolated, luminally perfused mouse stomach. Bombesin caused a concentration-dependent inhibition of basal and histamine-stimulated acid secretion and an increase in somatostatin secretion. Pertussis toxin was used to determine the contribution of somatostatin to the observed inhibition of acid secretion. Previous studies have shown that at a concentration of 125 ng/ml, pertussis toxin reverses completely the inhibition of acid secretion induced by exogenous and endogenous somatostatin. Preincubation of the stomach for 60 min with pertussis toxin (125 and 1250 ng/ml) reversed partially the inhibitory effect of bombesin on basal and histamine-stimulated acid secretion by 51%-59%, implying that bombesin-induced inhibition was partly mediated by release of somatostatin. The residual inhibition may represent a direct effect of bombesin on parietal cells.
在离体的、经腔内灌注的小鼠胃中研究了蛙皮素对胃酸分泌的作用及作用方式。蛙皮素引起基础胃酸分泌和组胺刺激的胃酸分泌呈浓度依赖性抑制，并使生长抑素分泌增加。使用百日咳毒素来确定生长抑素对所观察到的胃酸分泌抑制作用的贡献。先前的研究表明，在浓度为125 ng/ml时，百日咳毒素可完全逆转外源性和内源性生长抑素诱导的胃酸分泌抑制。用百日咳毒素（125和1250 ng/ml）对胃进行60分钟预孵育，可部分逆转蛙皮素对基础胃酸分泌和组胺刺激的胃酸分泌的抑制作用，逆转率为51%-59%，这意味着蛙皮素诱导的抑制作用部分是由生长抑素的释放介导的。残余的抑制作用可能代表蛙皮素对壁细胞的直接作用。
