Iwasa F, Furuya M, Hayashi Y, Ohnuma N
Laboratory of Clinical Pharmacology, Suntory Institute for Biomedical Research, Osaka.
J Biochem. 1989 May;105(5):697-9. doi: 10.1093/oxfordjournals.jbchem.a122729.
The effects of alpha-rat atrial natriuretic peptide (alpha-rANP) and sodium nitroprusside on the activity of rat lung particulate guanylate cyclase were examined. The particulate guanylate cyclase in partially purified rat lung membranes was stimulated by both alpha-rANP and nitroprusside. The effects of alpha-rANP and nitroprusside were, however, not additive. Diamide and N-ethylmaleimide almost completely abolished the nitroprusside-mediated stimulation, while they had only moderate effects on the alpha-rANP-mediated stimulation of the enzyme activity. ATP potentiated the enzyme stimulation by alpha-rANP, whereas it had no effect on the nitroprusside-mediated stimulation. These findings suggest that the stimulation of lung particulate guanylate cyclase activity by alpha-rANP and nitroprusside is mediated by different mechanisms.
研究了α-大鼠心房利钠肽（α-rANP）和硝普钠对大鼠肺微粒体鸟苷酸环化酶活性的影响。部分纯化的大鼠肺膜中的微粒体鸟苷酸环化酶受到α-rANP和硝普钠的刺激。然而，α-rANP和硝普钠的作用并非相加。二酰胺和N-乙基马来酰亚胺几乎完全消除了硝普钠介导的刺激，而它们对α-rANP介导的酶活性刺激只有中等程度的影响。ATP增强了α-rANP对酶的刺激作用，而对硝普钠介导的刺激没有影响。这些发现表明，α-rANP和硝普钠对肺微粒体鸟苷酸环化酶活性的刺激是由不同机制介导的。
