Talley Watts Lora, Long Justin Alexander, Manga Venkata Hemanth, Huang Shiliang, Shen Qiang, Duong Timothy Q
1] Research Imaging Institute, University of Texas Health Science Center, San Antonio, Texas, USA [2] Department of Cellular and Structure Biology, University of Texas Health Science Center, San Antonio, Texas, USA [3] Department of Neurology, University of Texas Health Science Center, San Antonio, Texas, USA.
Research Imaging Institute, University of Texas Health Science Center, San Antonio, Texas, USA.
J Cereb Blood Flow Metab. 2015 Jul;35(7):1137-44. doi: 10.1038/jcbfm.2015.18. Epub 2015 Feb 18.
Traumatic brain injury (TBI) is a multifaceted injury and a leading cause of death in children, young adults, and increasingly in Veterans. However, there are no neuroprotective agents clinically available to counteract damage or promote repair after brain trauma. This study investigated the neuroprotective effects of normobaric oxygen (NBO) after a controlled cortical impact in rats. The central hypothesis was that NBO treatment would reduce lesion volume and functional deficits compared with air-treated animals after TBI by increasing brain oxygenation thereby minimizing ischemic injury. In a randomized double-blinded design, animals received either NBO (n = 8) or normal air (n = 8) after TBI. Magnetic resonance imaging (MRI) was performed 0 to 3 hours, and 1, 2, 7, and 14 days after an impact to the primary forelimb somatosensory cortex. Behavioral assessments were performed before injury induction and before MRI scans on days 2, 7, and 14. Nissl staining was performed on day 14 to corroborate the lesion volume detected from MRI. Contrary to our hypothesis, we found that NBO treatment increased lesion volume in a rat model of moderate TBI and had no positive effect on behavioral measures. Our results do not promote the acute use of NBO in patients with moderate TBI.
创伤性脑损伤（TBI）是一种多方面的损伤，是儿童、年轻人乃至退伍军人死亡的主要原因。然而，目前临床上尚无神经保护剂可用于对抗脑外伤后的损伤或促进修复。本研究调查了常压氧（NBO）在大鼠控制性皮质撞击后的神经保护作用。核心假设是，与空气处理的动物相比，NBO治疗将通过增加脑氧合从而使缺血性损伤最小化，来减少TBI后大鼠的损伤体积和功能缺陷。在随机双盲设计中，动物在TBI后接受NBO（n = 8）或正常空气（n = 8）处理。在对初级前肢体感皮层进行撞击后的0至3小时、1天、2天以及7天和14天进行磁共振成像（MRI）检查。在损伤诱导前以及在第2天、第7天和第14天进行MRI扫描前进行行为评估。在第14天进行尼氏染色，以证实从MRI检测到的损伤体积。与我们的假设相反，我们发现在中度TBI大鼠模型中，NBO治疗增加了损伤体积，并且对行为指标没有积极影响。我们的结果不支持在中度TBI患者中急性使用NBO。
