Tamura Masayoshi, Mochizuki Naoki, Nagatomi Yasushi, Harayama Koichi, Toriba Akira, Hayakawa Kazuichi
Research Laboratories for Food Safety Chemistry, Asahi Group Holdings, Ltd., 1-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, Japan.
Graduate School of Medical Sciences, Kanazawa University, Kakuma-machi, Kanazawa-shi, Ishikawa 920-1192, Japan.
Toxins (Basel). 2015 Feb 16;7(2):582-92. doi: 10.3390/toxins7020582.
Three compounds, hypothesized as fumonisin A1 (FA1), fumonisin A2 (FA2), and fumonisin A3 (FA3), were detected in a corn sample contaminated with mycotoxins by high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS). One of them has been identified as FA1 synthesized by the acetylation of fumonisin B1 (FB1), and established a method for its quantification. Herein, we identified the two remaining compounds as FA2 and FA3, which were acetylated fumonisin B2 (FB2) and fumonisin B3 (FB3), respectively. Moreover, we examined a method for the simultaneous analysis of FA1, FA2, FA3, FB1, FB2, and FB3. The corn samples were prepared by extraction using a QuEChERS kit and purification using a multifunctional cartridge. The linearity, recovery, repeatability, limit of detection, and limit of quantification of the method were >0.99, 82.9%-104.6%, 3.7%-9.5%, 0.02-0.60 μg/kg, and 0.05-1.98 μg/kg, respectively. The simultaneous analysis of the six fumonisins revealed that FA1, FA2, and FA3 were present in all corn samples contaminated with FB1, FB2, and FB3. The results suggested that corn marketed for consumption can be considered as being contaminated with both the fumonisin B-series and with fumonisin A-series. This report presents the first identification and quantification of FA1, FA2, and FA3 in corn samples.
通过高分辨率液相色谱-轨道阱质谱（LC-Orbitrap MS）在一份被霉菌毒素污染的玉米样品中检测到三种化合物，推测为伏马菌素A1（FA1）、伏马菌素A2（FA2）和伏马菌素A3（FA3）。其中一种已被鉴定为通过伏马菌素B1（FB1）乙酰化合成的FA1，并建立了其定量方法。在此，我们将其余两种化合物鉴定为FA2和FA3，它们分别是乙酰化的伏马菌素B2（FB2）和伏马菌素B3（FB3）。此外，我们研究了一种同时分析FA1、FA2、FA3、FB1、FB2和FB3的方法。玉米样品通过使用QuEChERS试剂盒提取和多功能柱净化来制备。该方法的线性、回收率、重复性、检测限和定量限分别>0.99、82.9%-104.6%、3.7%-9.5%、0.02-0.60μg/kg和0.05-1.98μg/kg。对六种伏马菌素的同时分析表明，FA1、FA2和FA3存在于所有被FB1、FB2和FB3污染的玉米样品中。结果表明，投放市场供消费的玉米可被视为同时受到伏马菌素B系列和伏马菌素A系列的污染。本报告首次对玉米样品中的FA1、FA2和FA3进行了鉴定和定量。
