Bartók Tibor, Tölgyesi László, Szécsi Árpád, Mesterházy Ákos, Bartók Mihály, Gyimes Ernő, Véha Antal
Fumizol Ltd., Moszkvai krt. 5-7, H-6725 Szeged, Hungary
Eötvös Loránd University Joint Research and Training Laboratory on Separation Techniques, Pázmány Péter sétány 1/A, H-1117 Budapest, Hungary.
J Chromatogr Sci. 2014 Jul;52(6):508-13. doi: 10.1093/chromsci/bmt071. Epub 2013 Jun 7.
Five previously unknown fumonisin mycotoxins (iso-FP1, iso-FP(2,3a), iso-FP(2,3b), FP4 and iso-FP4) and three previously described FP analogues (FP(1-3)) were detected in a solid rice culture inoculated with Fusarium verticillioides. The fumonisins were characterized by high-performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry (HPLC-ESI-TOFMS) and ion trap mass spectrometry (ITMS). The FP isomers were separated by using a flat gradient on a special, high-coverage C18, narrow-bore HPLC column (YMC-Pack J'sphere ODS H80), which was suggested for the separation of structural isomers. The verified structures of the FP(1-3) mycotoxins, the relative retention times (by HPLC-ESI-TOFMS and ITMS), the exact masses of the molecular ions (by TOFMS) and the masses of the product ions, including the hydrocarbon backbones (by ITMS) of the new compounds, strongly suggested their structures.
在用轮枝镰孢接种的水稻固体培养物中检测到了5种先前未知的伏马菌素霉菌毒素（异-FP1、异-FP(2,3a)、异-FP(2,3b)、FP4和异-FP4）以及3种先前已描述的FP类似物（FP(1-3)）。通过高效液相色谱-电喷雾电离飞行时间质谱法（HPLC-ESI-TOFMS）和离子阱质谱法（ITMS）对伏马菌素进行了表征。使用特殊的高覆盖C18窄孔HPLC柱（YMC-Pack J'sphere ODS H80）上的平梯度分离FP异构体，该柱建议用于结构异构体的分离。FP(1-3)霉菌毒素的已验证结构、相对保留时间（通过HPLC-ESI-TOFMS和ITMS）、分子离子的精确质量（通过TOFMS）以及产物离子的质量，包括新化合物的碳氢骨架（通过ITMS），有力地表明了它们的结构。
