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用于小基因组的生物信息学工具，如乙型肝炎病毒。

Bioinformatics tools for small genomes, such as hepatitis B virus.

作者信息

Bell Trevor G, Kramvis Anna

机构信息

Hepatitis Virus Diversity Research Programme (HVDRP), Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2050, South Africa.

出版信息

Viruses. 2015 Feb 16;7(2):781-97. doi: 10.3390/v7020781.

DOI:10.3390/v7020781
PMID:25690798
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4353916/
Abstract

DNA sequence analysis is undertaken in many biological research laboratories. The workflow consists of several steps involving the bioinformatic processing of biological data. We have developed a suite of web-based online bioinformatic tools to assist with processing, analysis and curation of DNA sequence data. Most of these tools are genome-agnostic, with two tools specifically designed for hepatitis B virus sequence data. Tools in the suite are able to process sequence data from Sanger sequencing, ultra-deep amplicon resequencing (pyrosequencing) and chromatograph (trace files), as appropriate. The tools are available online at no cost and are aimed at researchers without specialist technical computer knowledge. The tools can be accessed at http://hvdr.bioinf.wits.ac.za/SmallGenomeTools, and the source code is available online at https://github.com/DrTrevorBell/SmallGenomeTools.

摘要

许多生物学研究实验室都进行DNA序列分析。该工作流程包括几个步骤，涉及生物数据的生物信息学处理。我们开发了一套基于网络的在线生物信息学工具，以协助处理、分析和管理DNA序列数据。这些工具大多与基因组无关，其中有两个工具是专门为乙肝病毒序列数据设计的。该工具套件中的工具能够根据需要处理来自桑格测序、超深度扩增子重测序（焦磷酸测序）和色谱图（跟踪文件）的序列数据。这些工具可免费在线获取，面向没有专业技术计算机知识的研究人员。可通过http://hvdr.bioinf.wits.ac.za/SmallGenomeTools访问这些工具，其源代码可在https://github.com/DrTrevorBell/SmallGenomeTools上在线获取。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/0e180626018a/viruses-07-00781-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/08a9cbe4a786/viruses-07-00781-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/b0b78fdc58e6/viruses-07-00781-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/dd1346b71794/viruses-07-00781-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/a4abd4cdf62b/viruses-07-00781-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/c8496c1c7675/viruses-07-00781-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/027151e3117d/viruses-07-00781-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/0db8b203796f/viruses-07-00781-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/140c4f88768f/viruses-07-00781-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/89de55d2de7f/viruses-07-00781-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/0e180626018a/viruses-07-00781-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/08a9cbe4a786/viruses-07-00781-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/b0b78fdc58e6/viruses-07-00781-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/dd1346b71794/viruses-07-00781-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/a4abd4cdf62b/viruses-07-00781-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/c8496c1c7675/viruses-07-00781-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/027151e3117d/viruses-07-00781-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/0db8b203796f/viruses-07-00781-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/140c4f88768f/viruses-07-00781-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/89de55d2de7f/viruses-07-00781-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e379/4353916/0e180626018a/viruses-07-00781-g010.jpg

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