Singh Ruchi, Mishra Anand, Dhawan Sunita S, Shirke Pramod A, Gupta Madan M, Sharma Ashok
Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
Plant Physiology Division, CSIR-National Botanical Research Institute, Lucknow, India.
Protoplasma. 2015 Nov;252(6):1439-50. doi: 10.1007/s00709-015-0771-z. Epub 2015 Feb 19.
Physiological, biochemical, and gene expression responses under drought stress were studied in Withania somnifera. Photosynthesis rate, stomatal conductance, transpiration rate, relative water content, chlorophyll content, and quantum yield of photosystems I and II (PSI and PSII) decreased in response to drought stress. Comparative expression of genes involved in osmoregulation, detoxification, signal transduction, metabolism, and transcription factor was analyzed through quantitative RT-PCR. The genes encoding 1-pyrroline-5-carboxylate synthetase (P5CS), glutathione S-transferase (GST), superoxide dismutase (SOD), serine threonine-protein kinase (STK), serine threonine protein phosphatase (PSP), aldehyde dehydrogenase (AD), leucoanthocyanidin dioxygenase/anthocyanin synthase (LD/AS), HSP, MYB, and WRKY have shown upregulation in response to drought stress condition in leaf tissues. Enhanced detoxification and osmoregulation along with increased withanolides production were also observed under drought stress. The results of this study will be helpful in developing stress-tolerant and high secondary metabolite yielding genotypes.
对印度人参在干旱胁迫下的生理、生化和基因表达反应进行了研究。干旱胁迫下，光合作用速率、气孔导度、蒸腾速率、相对含水量、叶绿素含量以及光系统I和II（PSI和PSII）的量子产率均下降。通过定量RT-PCR分析了参与渗透调节、解毒、信号转导、代谢和转录因子的基因的相对表达。编码1-吡咯啉-5-羧酸合成酶（P5CS）、谷胱甘肽S-转移酶（GST）、超氧化物歧化酶（SOD）、丝氨酸苏氨酸蛋白激酶（STK）、丝氨酸苏氨酸蛋白磷酸酶（PSP）、醛脱氢酶（AD）、无色花青素双加氧酶/花青素合酶（LD/AS）、热休克蛋白（HSP）、MYB和WRKY的基因在叶片组织干旱胁迫条件下表现出上调。在干旱胁迫下还观察到解毒和渗透调节增强以及醉茄内酯产量增加。本研究结果将有助于培育耐胁迫和高次生代谢产物产量的基因型。
