利用磁珠生物反应器和液相色谱-串联质谱法表征活性代谢物引起的蛋白质修饰。
Characterizing protein modifications by reactive metabolites using magnetic bead bioreactors and LC-MS/MS.
作者信息
Li Dandan, Fu You-Jun, Rusling James F
机构信息
Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269, USA.
出版信息
Chem Commun (Camb). 2015 Mar 18;51(22):4701-3. doi: 10.1039/c5cc00420a.
Abstract
We report here label-free metabolite-protein adduct detection and identification employing magnetic beads coated with metabolic enzymes as bioreactors to generate metabolites and possible metabolite-protein adducts for analysis by liquid chromatography-tandem mass spectrometry.
摘要
我们在此报告了一种无标记代谢物-蛋白质加合物的检测与鉴定方法，该方法利用涂有代谢酶的磁珠作为生物反应器来生成代谢物以及可能的代谢物-蛋白质加合物，以供液相色谱-串联质谱分析。
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