Tan Chong, Liu Ying-Jie, Wang Wei, Qiu Shan, Ma Fang
Huan Jing Ke Xue. 2014 Oct;35(10):3807-13.
The short-cut nitrification and denitrification process of a polyurethane biofilm reactor was studied at four different carbon to nitrogen ratios (C/N ratios) (C/N =15:1, 10:1, 5:1 and 1.8:1) with focus on reactor performance and microbial community composition. Results showed that when the influent carbon/nitrogen ratio decreased from 15 to 10, 5 and 1.8, the nitrite accumulation rate was increased from 56.1% to 62.3%, 72.3% and 83.2% after 100 d operation. System achieved the optimal removal efficiency at an influent carbon/nitrogen ratio of 10 with 99.1% of ammonia removal and 91.0% of total nitrogen removal. Simultaneous nitrification and denitrification occurred in the polyurethane biofilm system at different influent carbon/nitrogen ratio. As the influent carbon/nitrogen ratio decreased from 15 to 10, 5 and 1.8, the efficiency of simultaneous nitrification and denitrification was gradually reduced. The microbial biofilm analysis indicated that the microbial diversity of biofilm with an influent carbon/nitrogen ratio of 15 was significantly higher than that in other conditions. The predominant ammonia oxidizing bacteria and nitrite oxidizing bacteria were Nitrosospira sp. and Nitrospira sp., respectively, and the predominant denitrifying bacteria were Pseudomonas sp.
在四个不同的碳氮比（C/N比）（C/N = 15:1、10:1、5:1和1.8:1）条件下，研究了聚氨酯生物膜反应器的短程硝化反硝化过程，重点关注反应器性能和微生物群落组成。结果表明，当进水碳氮比从15降至10、5和1.8时，运行100 d后，亚硝酸盐积累率从56.1%提高到62.3%、72.3%和83.2%。进水碳氮比为10时，系统实现了最佳去除效率，氨氮去除率为99.1%，总氮去除率为91.0%。在不同进水碳氮比条件下，聚氨酯生物膜系统中均发生了同步硝化反硝化反应。随着进水碳氮比从15降至10、5和1.8，同步硝化反硝化效率逐渐降低。微生物生物膜分析表明，进水碳氮比为15时生物膜的微生物多样性显著高于其他条件。主要的氨氧化细菌和亚硝酸盐氧化细菌分别为亚硝化螺菌属（Nitrosospira sp.）和硝化螺菌属（Nitrospira sp.），主要的反硝化细菌为假单胞菌属（Pseudomonas sp.）。
