Forsyth K D, Simpson A C, Levinsky R J
Department of Immunology, Institute of Child Health, University of London.
Eur J Immunol. 1989 Jul;19(7):1331-4. doi: 10.1002/eji.1830190727.
Anti-neutrophil antibodies (CD15) bind to a simple sugar (lactose-N-fucopentaose III, LNF III) known to be present on the chains of the adhesion molecules on neutrophils. We have demonstrated that pre-incubation of neutrophils with a CD15 antibody increases neutrophil adherence to endothelium by a neutrophil-dependent mechanism. This augmented adhesion can be inhibited by antibodies directed against the alpha and beta chain of the leukocyte function-associated antigen 1 (LFA-1) molecule. There is also some increase in surface LFA-1 expression on neutrophils after CD15 incubation, suggesting that CD15 antibodies increase neutrophil adhesion by an LFA-1-dependent mechanism. The increase in LFA-1 expression after CD15 incubation occurs in the presence of a protein synthesis inhibitor. As LFA-1 is not stored intracellularly, the increased adherence of the neutrophils, and increased LFA-1 expression on their surface, suggests the possibility that the CD15 antibodies are binding to the LNF III antigen present on the alpha and beta chains of LFA-1, producing their effect by activating the molecule, perhaps by exposing new antigen sites by a stearic effect.
抗中性粒细胞抗体（CD15）与一种已知存在于中性粒细胞黏附分子链上的单糖（乳糖-N-岩藻五糖III，LNF III）结合。我们已经证明，用CD15抗体预孵育中性粒细胞会通过一种中性粒细胞依赖的机制增加中性粒细胞与内皮细胞的黏附。这种增强的黏附可以被针对白细胞功能相关抗原1（LFA-1）分子的α链和β链的抗体所抑制。在CD15孵育后，中性粒细胞表面的LFA-1表达也有一定增加，这表明CD15抗体通过一种LFA-1依赖的机制增加中性粒细胞黏附。在存在蛋白质合成抑制剂的情况下，CD15孵育后LFA-1表达仍会增加。由于LFA-1不储存在细胞内，中性粒细胞黏附增加以及其表面LFA-1表达增加，提示CD15抗体可能与LFA-1的α链和β链上存在的LNF III抗原结合，通过激活该分子产生作用，也许是通过空间效应暴露新的抗原位点。
