配对蛋白的RED结构域是果蝇附腺成熟所特别需要的。

The RED domain of Paired is specifically required for Drosophila accessory gland maturation.

作者信息

Li Li, Li Ping, Xue Lei

机构信息

Institute of Intervention Vessel, Shanghai 10th People's Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Science and Technology, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China.

出版信息

Open Biol. 2015 Feb;5(2):140179. doi: 10.1098/rsob.140179.

DOI:10.1098/rsob.140179

PMID:25694546

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4345280/

Abstract

The evolutionarily conserved paired domain consists of the N-terminal PAI and the C-terminal RED domains, each containing a helix-turn-helix motif capable of binding DNA. Despite its conserved sequence, the physiological functions of the RED domain remain elusive. Here, we constructed a prd transgene expressing a truncated Paired (Prd) protein without the RED domain, and examined its rescue ability in prd mutants. We found that the RED domain is specifically required for the expression of Acp26Aa and sex peptide in male accessory glands, and the induction of female post-mating response. Our data thus identified an important physiological function for the evolutionarily conserved RED domain.

摘要

进化上保守的配对结构域由N端的PAI结构域和C端的RED结构域组成，每个结构域都包含一个能够结合DNA的螺旋-转角-螺旋基序。尽管RED结构域的序列保守，但其生理功能仍不清楚。在这里，我们构建了一个表达缺失RED结构域的截短配对（Prd）蛋白的prd转基因，并检测了其在prd突变体中的拯救能力。我们发现，RED结构域对于雄性附腺中Acp26Aa和性肽的表达以及雌性交配后反应的诱导是特异性必需的。因此，我们的数据确定了进化上保守的RED结构域的一个重要生理功能。