Friedlander M L, Bell D R, Leary J, Davey R A
Department of Clinical Oncology, Royal North Shore Hospital, St Leonards, New South Wales, Australia.
J Clin Pathol. 1989 Jul;42(7):719-22. doi: 10.1136/jcp.42.7.719.
A sensitive immunocytochemical technique was developed to detect a 170,000 dalton cell membrane glycoprotein (P-gp) in cell lines resistant to vincristine and vinblastine with varying degrees of resistance. P-gp was shown very clearly using the C219 monoclonal antibody and immunocytochemical detection with either antialkaline phosphate or peroxidase-antiperoxidase with silver gold intensification. There was good correlation between the results obtained with immunocytochemical detection of P-gp in single cells and Western blot analysis. The technique is easily performed and can detect P-gp in relatively small numbers of cells that Western blot analysis could miss and is suitable for clinical application.
开发了一种灵敏的免疫细胞化学技术，用于检测对长春新碱和长春花碱具有不同程度抗性的细胞系中的一种170,000道尔顿的细胞膜糖蛋白（P-糖蛋白）。使用C219单克隆抗体以及抗碱性磷酸酶或过氧化物酶-抗过氧化物酶并经银金强化的免疫细胞化学检测，P-糖蛋白显示得非常清晰。单细胞中P-糖蛋白的免疫细胞化学检测结果与蛋白质印迹分析结果之间具有良好的相关性。该技术操作简便，能够检测出蛋白质印迹分析可能遗漏的相对少量细胞中的P-糖蛋白，适用于临床应用。
