Rahimi Hamzeh, Farajollahi Mohammad M, Hosseini Arshad
1. PhD student of Department of Medical Biotechnology, Iranian Pasteur Institute, Pasteur Square, Tehran, Iran.
2. Professor of Department of Medical Biotechnology, Faculty of Allied Medicine & Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran & Alborz Food & Drug Laboratory, Fardis, Karaj, Alborz, Iran.
Med J Islam Repub Iran. 2014 Nov 29;28:140. eCollection 2014.
The CCR5 is a chemokine receptor that serves as a co-receptor for HIV-1 attachment and entry to T lymphocytes. A 32bp deletion (∆32) in this gene is believed to be associated with resistance to infection and delay disease progression. The aim of this study was to determine the∆32 allele frequency in healthy individuals and HIV-infected individuals with AIDS.
In this experiment, 530 normal individuals from healthy Iranian population and 40 HIV-infected samples from Western Clinic of Tehran were examined for∆32 in CCR5 gene using polymerase chain reaction (PCR) techniques followed by agarose gel electrophoresis.
Allele frequencies of the CCR5∆32 in normal individuals were calculated to be 1.1% for heterozygous genotype and 0.19% for homozygous genotype. None of the co-receptor gene in HIV cases was found to be mutated in this study.
Based on the findings of this study and the literature in Iran, we could conclude that Iranian people similar to neighbor countries such as Arabs are susceptible to HIV virus infection.
CCR5是一种趋化因子受体，作为HIV-1附着并进入T淋巴细胞的共受体。该基因中的一个32bp缺失（∆32）被认为与抗感染和延缓疾病进展有关。本研究的目的是确定健康个体和患有艾滋病的HIV感染者中∆32等位基因频率。
在本实验中，使用聚合酶链反应（PCR）技术，随后进行琼脂糖凝胶电泳，对来自伊朗健康人群的530名正常个体和来自德黑兰西部诊所的40份HIV感染样本进行CCR5基因∆32检测。
正常个体中CCR5∆32的等位基因频率计算得出，杂合基因型为1.1%，纯合基因型为0.19%。本研究中未发现HIV病例的共受体基因发生突变。
基于本研究的结果以及伊朗的文献，我们可以得出结论，伊朗人与阿拉伯等邻国一样，容易感染HIV病毒。
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