Li C, Yan Y P, Shieh B, Lee C M, Lin R Y, Chen Y M
Department of Pathology, National Cheng Kung University Medical College, Tainan, Taipei, Taiwan.
J Formos Med Assoc. 1997 Dec;96(12):979-84.
A specific 32-nucleotide deletion mutant of the CCR5 gene (Accr5), the coreceptor gene for human immunodeficiency virus type 1 (HIV-1), can effectively suppress the transmission and pathogenesis of the virus. Individuals homozygous for the delta ccr5 allele resist primary macrophage-tropic HIV-1 infection, despite multiple high-risk sexual exposures. This gene deletion is relatively common among Caucasians but uncommon among Africans, Asians, and South Americans. We used polymerase chain reaction (PCR) technology to determine the frequency of the delta ccr5 allele in a Taiwanese population with diverse health status and social backgrounds. Subjects included 24 HIV-1-infected persons in the northern and southern parts of Taiwan; 131 HIV-1 high-risk, licensed female sex workers in the northern part of the island (21% of whom were aborigines); and 187 unrelated, healthy, HIV-1-negative individuals in southern Taiwan. PCR with primers encompassing the entire CCR5 gene was used to explore possible deletions at regions other than the 32-nucleotide area in the female sex workers. No ccr5 deletions were detected, indicating that they are rare or absent in the Taiwanese population. This finding implies that delta ccr5 is not likely to be part of the defense against the spread of HIV-1-infection in Taiwanese.
人免疫缺陷病毒1型（HIV-1）的共受体基因CCR5的一种特定的32个核苷酸缺失突变体（Accr5）能够有效抑制该病毒的传播和发病机制。尽管有多次高危性接触，但纯合携带δccr5等位基因的个体可抵抗原发性巨噬细胞嗜性HIV-1感染。这种基因缺失在高加索人中相对常见，但在非洲人、亚洲人和南美洲人中并不常见。我们使用聚合酶链反应（PCR）技术来确定具有不同健康状况和社会背景的台湾人群中δccr5等位基因的频率。研究对象包括台湾北部和南部的24名HIV-1感染者；台湾北部131名HIV-1高危、持牌女性性工作者（其中21%为原住民）；以及台湾南部187名无亲缘关系、健康的HIV-1阴性个体。使用包含整个CCR5基因的引物进行PCR，以探究女性性工作者中除32个核苷酸区域以外其他区域可能存在的缺失情况。未检测到ccr5缺失，表明它们在台湾人群中罕见或不存在。这一发现意味着δccr5不太可能是台湾人群抵御HIV-1感染传播的防御机制的一部分。
