一种用于检测还原型谷胱甘肽的有效电化学生物传感平台。
An effective electrochemical biosensing platform for the detection of reduced glutathione.
机构信息
a Department of Chemistry , Mugla Sitki Kocman University, Faculty of Science , Kötekli, Mugla , Turkiye.
b Department of Chemistry , Ege University, Faculty of Science , Bornova, Izmir , Turkiye.
出版信息
Artif Cells Nanomed Biotechnol. 2016 May;44(3):971-7. doi: 10.3109/21691401.2015.1008504. Epub 2015 Feb 20.
A biosensor was developed using glutathione peroxidase (GSH-Px) as the enzyme. Firstly, platinum (Pt) nanoparticles were deposited onto a glassy carbon paste electrode (GCPE), and then GSH-Px was immobilized by means of gelatin that was then crosslinked with glutaraldehyde. The measurement was based on the electrochemical oxidation of GSH to its disulfide form in the presence of hydrogen peroxide. The linear range was found to be between 10 and 250 μM, with a correlation coefficient of R(2) = 0.9968. The R.S.D value for 25 μM GSH (n = 6) was calculated as 2.92%. Finally, the proposed biosensor was used to analyze GSH in a synthetically prepared plasma sample, and a promising recovery value was obtained.
研制了一种基于谷胱甘肽过氧化物酶（GSH-Px）的生物传感器。首先，将铂（Pt）纳米粒子沉积在玻碳糊电极（GCPE）上，然后通过明胶固定 GSH-Px，然后用戊二醛交联。测量是基于在存在过氧化氢的情况下将 GSH 电化学氧化为其二硫化物形式。发现线性范围在 10 到 250 μM 之间，相关系数 R(2) = 0.9968。计算 25 μM GSH（n = 6）的 R.S.D 值为 2.92%。最后，该生物传感器用于分析合成制备的血浆样品中的 GSH，获得了有前途的回收率值。
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