Conrad P A, ole-MoiYoi O K, Baldwin C L, Dolan T T, O'Callaghan C J, Njamunggeh R E, Grootenhuis J G, Stagg D A, Leitch B L, Young A S
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Parasitology. 1989 Apr;98 Pt 2:179-88. doi: 10.1017/s0031182000062089.
The characteristics of intra-lymphocytic Theileria isolated from African buffalo and from cattle that were infected with buffalo-derived parasites were evaluated using anti-schizont monoclonal antibodies (mAbs) and DNA probes. Antigenic differences were revealed by the reactivities of 27 mAbs with the buffalo-derived parasites isolated from different animals. Antigenic diversity was also seen with Theileria-infected lymphoblastoid cell isolates taken from the lymph nodes and lambda gt11, showed specific hybridization to parasite DNA in Southern blots of restriction enzyme-digested, lymphoblastoid cells infected with buffalo-derived theilerial parasites. Genotypic differences between the buffalo-derived parasites were revealed by the restriction fragment length polymorphisms seen with hybridization of those probes to DNA from cloned and uncloned Theileria-infected cell lines. The evaluation of theilerial parasites derived from buffalo and from cattle which underwent typical T. p. lawrencei reactions, after being infected with buffalo-derived theilerial parasites, did not show any specific phenotypic or genotypic characteristics of these parasites that would distinguish them from T. p. parva and T. p. bovis parasites. The validity of these subspecies distinctions is discussed.
利用抗裂殖体单克隆抗体（mAb）和DNA探针，对从非洲水牛以及感染了源自水牛的寄生虫的牛体内分离出的淋巴细胞内泰勒虫的特征进行了评估。通过27种单克隆抗体与从不同动物分离出的源自水牛的寄生虫的反应性，揭示了抗原差异。从淋巴结获取的感染泰勒虫的淋巴母细胞样细胞分离株也呈现出抗原多样性，并且λgt11在经限制性内切酶消化的、感染了源自水牛的泰勒虫寄生虫的淋巴母细胞样细胞的Southern印迹中，与寄生虫DNA显示出特异性杂交。通过这些探针与来自克隆和未克隆的感染泰勒虫的细胞系的DNA杂交所观察到的限制性片段长度多态性，揭示了源自水牛的寄生虫之间的基因型差异。对感染了源自水牛的泰勒虫寄生虫后出现典型T. p. lawrencei反应的水牛和牛的泰勒虫寄生虫进行评估，未发现这些寄生虫有任何特定的表型或基因型特征可将它们与T. p. parva和T. p. bovis寄生虫区分开来。讨论了这些亚种区分的有效性。
