Najafi Mehdi, Calvert Peter D
Department of Ophthalmology and the Center for Vision Research, State University of New York Upstate Medical University, Syracuse, NY, USA.
Methods Mol Biol. 2015;1271:309-23. doi: 10.1007/978-1-4939-2330-4_20.
High-resolution multiphoton imaging of live cells has become an invaluable method to study protein dynamics in highly compartmentalized subcellular environments. Here we describe procedures that we recently developed to quantify rhodopsin mobility within and between retinal rod photoreceptor light signaling microcompartments, the disc membrane lobules, using multiphoton fluorescence relaxation after photoconversion.
活细胞的高分辨率多光子成像已成为研究高度区室化亚细胞环境中蛋白质动力学的一种极其重要的方法。在此，我们描述了我们最近开发的程序，该程序利用光转化后的多光子荧光弛豫来量化视紫红质在视网膜视杆光感受器光信号微区室（盘膜小叶）内以及之间的流动性。
