Yang Z W, Paleos G A, Byrd J C
Developmental Neurobiology Program, University of Pittsburgh Center for Neuroscience, PA 15213.
Eur J Pharmacol. 1989 May 30;164(3):607-10. doi: 10.1016/0014-2999(89)90274-4.
Phencyclidine binds with high affinity to both PCP and sigma receptors. We investigated whether the clonal cell line PC12 expressed either of these receptors, and found that these cells contain a haloperidol-sensitive (+)-[3H]3-PPP binding site with a KD of 56 nM, but no PCP binding sites. The (+)3-PPP binding sites in PC12 cells displayed a reversed stereoselectivity for the benzomorphan opiates compared to CNS sigma receptors. Neither nerve growth factor nor sodium butyrate treatment affected the expression of either (+)-3-PPP or TCP binding sites in PC12 cells.
苯环利定与苯环己哌啶受体和σ受体都具有高亲和力结合。我们研究了克隆细胞系PC12是否表达这些受体中的任何一种，发现这些细胞含有一个对氟哌啶醇敏感的（+）-[³H]3-PPP结合位点，解离常数为56 nM，但没有苯环己哌啶结合位点。与中枢神经系统σ受体相比，PC12细胞中的（+）-3-PPP结合位点对苯吗喃类阿片显示出相反的立体选择性。神经生长因子和丁酸钠处理均未影响PC12细胞中（+）-3-PPP或TCP结合位点的表达。
