Fukuishi N, Gemba M
Division of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.
Jpn J Pharmacol. 1989 Jun;50(2):247-9. doi: 10.1254/jjp.50.247.
The nephrotoxicity of cisplatin was evaluated in an in vitro system with an established cell line of normal rat kidney, NRK-52E. Leakage of enzymes from the cells into the culture medium increased when they were exposed to 1 microM of cisplatin for 72 hr. The level of lipid peroxides increased in the cells after 48 hr of exposure to cisplatin; the increase was more rapid than the enzyme leakage. This culture system can be used to evaluate drug-induced nephrotoxicity and its mechanism.
在体外系统中，利用已建立的正常大鼠肾细胞系NRK-52E评估了顺铂的肾毒性。当细胞暴露于1微摩尔的顺铂72小时时，酶从细胞泄漏到培养基中的量增加。在暴露于顺铂48小时后，细胞内脂质过氧化物水平升高；这种升高比酶泄漏更快。该培养系统可用于评估药物诱导的肾毒性及其机制。
