Nakamura Makoto, Jinnai Wataru, Hamano Satoshi, Nakamura Shinji, Koyano Kosuke, Chiba Yoichi, Kanenishi Kenji, Yasuda Saneyuki, Ueno Masaki, Miki Takanori, Hata Toshiyuki, Kusaka Takashi
Department of Pediatrics, Faculty of Medicine, Kagawa University, Kagawa, Japan; Department of Neonatology, National Hospital Organization Okayama Medical Center, Okayama, Japan.
Maternal Perinatal Center, Faculty of Medicine, Kagawa University, Kagawa, Japan.
Int J Dev Neurosci. 2015 May;42:1-9. doi: 10.1016/j.ijdevneu.2015.02.009. Epub 2015 Feb 19.
The aim of this study was to assess the relationship between the cerebral blood volume (CBV) measured by near-infrared time-resolved spectroscopy (TRS) and pathological change of the brain in a hypoxic-ischemic (HI) piglet model. Twenty-one anesthetized newborn piglets, including three sham controls, were studied. An HI event was induced by low inspired oxygen. CBV was measured using TRS (Hamamatsu TRS-10). Data were collected before, during, and 6h after the insult. CBV was calculated as the change from the end of the insult. The piglets were allowed to recover from anesthesia for 6h after the insult. At the age of 5 days, the brains of the piglets were perfusion-fixed, and histologic evaluations of brain tissue were performed. The extent of histopathological damage was graded in 0.5-unit intervals on a 9-step scale. CBV increments were well correlated with histopathological scores, especially at 1 and 3h after resuscitation. Spearman's rank-correlation coefficients at 1, 3, and 6h after resuscitation in the gray matter were 0.9016, 0.9127, and 0.6907, respectively. We conclude that an increased CBV after HI insult indicates more marked histological brain damage. CBV measurement immediately after resuscitation provides a more precise prediction of the histological outcome.
本研究的目的是评估在缺氧缺血（HI）仔猪模型中，通过近红外时间分辨光谱法（TRS）测量的脑血容量（CBV）与脑病理变化之间的关系。对21只麻醉的新生仔猪进行了研究，其中包括3只假手术对照组。通过低吸入氧诱导HI事件。使用TRS（滨松TRS-10）测量CBV。在损伤前、损伤期间和损伤后6小时收集数据。CBV计算为损伤结束后的变化量。损伤后让仔猪从麻醉中恢复6小时。在5日龄时，对仔猪的大脑进行灌注固定，并对脑组织进行组织学评估。组织病理学损伤程度按9级量表以0.5单位间隔分级。CBV增加与组织病理学评分密切相关，尤其是在复苏后1小时和3小时。复苏后1小时、3小时和6小时灰质中的Spearman等级相关系数分别为0.9016、0.9127和0.6907。我们得出结论，HI损伤后脑血容量增加表明脑组织学损伤更明显。复苏后立即测量CBV能更精确地预测组织学结果。
