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轮状病毒在爱尔兰流行株型的变化:G2P[4]的再次出现和在爱尔兰鉴定出新基因型。

Changing patterns of rotavirus strains circulating in Ireland: re-emergence of G2P[4] and identification of novel genotypes in Ireland.

机构信息

Department of Biological Sciences, Cork Institute of Technology, Cork, Ireland.

出版信息

J Med Virol. 2015 May;87(5):764-73. doi: 10.1002/jmv.24095. Epub 2015 Feb 20.

Abstract

Worldwide, Group A Rotavirus (RVA) is recognized as the most common aetiological agent of acute diarrheal disease in children. One hundred and ninety seven positive faecal samples were obtained from patients between 2006 and 2008. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to amplify the VP7 and VP4 gene segments of these samples, and G and P typing was carried out subsequently. The most common strain type was G1P[8], and the emergent global G9-type was identified in both years. RVA strain type G2P[4], previously reported in Ireland in 1999, was also detected. Genotypes G2 and G3 in combination with P[4] were detected in 2006-2007 only. There was also an emergence of strain types including G3P[4], G9P[4], G2P[4 + 8] and G2G4P[8] in this study. Molecular analysis of the VP7 genes revealed G1 strains circulating within lineage Ic as previously reported in Ireland. In addition, new sublineage within lineage I of G1 strains was also identified. Analysis of G4 strain NRVL-Hum-49 revealed similarity with other human G4 viruses in lineage Ib. G9 strain NRVL-Hum-74 clustered with a unique G9 strain, CIT-254, in lineage IIIc. This data supports the observations made that the profile of RVA strains in Ireland appears to be dynamic. This study demonstrates that the circulation of human rotavirus is changing continuously in Ireland, and continued surveillance of the circulating strains is needed to detect the appearance of new strains, or new variants which may lead to vaccine breakthrough.

摘要

在全球范围内,A 组轮状病毒(RVA)被认为是导致儿童急性腹泻病的最常见病因。我们从 2006 年至 2008 年的患者中获得了 197 份阳性粪便样本。使用逆转录聚合酶链反应(RT-PCR)扩增这些样本的 VP7 和 VP4 基因片段,随后进行 G 和 P 型分型。最常见的菌株类型是 G1P[8],并且在这两年中都发现了新兴的全球 G9 型。还检测到了以前在 1999 年爱尔兰报道过的 RVA 菌株 G2P[4]。仅在 2006-2007 年检测到 G2 和 G3 与 P[4]的组合。在这项研究中还出现了包括 G3P[4]、G9P[4]、G2P[4+8]和 G2G4P[8]在内的菌株类型。VP7 基因的分子分析显示,与之前在爱尔兰报道的一样,Ic 谱系内存在 G1 株。此外,还确定了 G1 株的 I 谱系内的新亚谱系。对 G4 株 NRVL-Hum-49 的分析表明,与 Ib 谱系中的其他人类 G4 病毒具有相似性。G9 株 NRVL-Hum-74 与独特的 G9 株 CIT-254 聚类在 IIIc 谱系中。这些数据支持了这样的观察结果,即爱尔兰的 RVA 菌株谱似乎是动态的。本研究表明,人类轮状病毒在爱尔兰的传播不断变化,需要对循环株进行持续监测,以发现新菌株的出现,或可能导致疫苗突破的新变体。

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