National Center for Immunobiologicals Research and Evaluation, Istituto Superiore di Sanità, Rome, Italy.
J Med Virol. 2014 Jun;86(6):1073-82. doi: 10.1002/jmv.23871. Epub 2014 Jan 10.
Hospital-based surveillance of acute gastroenteritis caused by rotavirus has produced ample knowledge on the infection in children, whereas little is known on rotavirus infection among adults. The Italian surveillance program RotaNet-Italia collected 1,595 samples from patients admitted to hospital with gastroenteritis in 2012. All patients presented with vomiting, diarrhea, fever, and/or abdominal pain. Forty-two samples obtained by the RotaNet-Italia (2.6%) were from adolescents or adults (10-89 years). The study compared the genotypes and gene sequences of the rotavirus strains identified in adults with strains obtained from children worldwide. All 42 Italian strains were genotyped by the EuroRotaNet RT-nested-PCR protocols, and 12 rotaviruses from patients >13-year-old were subjected to nucleotide sequencing of their VP7 and/or VP4 genes. All strains analyzed belonged to the common human genotypes G1P[8], G2P[4], G4P[8], and G9P[8], except an uncommon G3P[19] genotype detected in a single patient. Phylogenetic analysis of the 12 strains showed that within each genotype they clustered in RVA lineages reported worldwide. The amino acid sequences of the VP7 and the VP8* hypervariable regions were highly conserved between the RVA strains collected from adults and children, in each lineage. Genotyping, phylogenetic analysis, and the study of viral epitopes revealed that rotaviruses circulating in adults in Italy are closely similar to the strains circulating in children, with high nucleotide identity particularly with strains reported in Europe and Asia. The circulation of the same rotavirus strains in both children and adults suggests that adults may contribute to sustain the circulation of rotaviruses through the population.
基于医院的轮状病毒急性胃肠炎监测为儿童感染提供了丰富的知识，而成年人的轮状病毒感染则知之甚少。意大利的 RotaNet-Italia 监测项目于 2012 年从因胃肠炎住院的患者中收集了 1595 个样本。所有患者均出现呕吐、腹泻、发热和/或腹痛。RotaNet-Italia 获得的 42 个样本来自青少年或成年人（10-89 岁）。该研究比较了成年人和世界各地儿童中分离出的轮状病毒株的基因型和基因序列。所有 42 株意大利分离株均通过 EuroRotaNet RT-巢式 PCR 方案进行基因分型，12 株来自 13 岁以上患者的轮状病毒进行其 VP7 和/或 VP4 基因的核苷酸测序。所有分析的菌株均属于常见的人类基因型 G1P[8]、G2P[4]、G4P[8]和 G9P[8]，除了在单个患者中检测到的一种罕见的 G3P[19]基因型。12 株病毒的系统进化分析表明，在每个基因型内，它们均聚类为在世界范围内报道的 RVA 谱系。在每个谱系中，成人和儿童分离株的 VP7 和 VP8*高变区的氨基酸序列高度保守。基因型、系统进化分析和病毒表位研究表明，意大利成人中流行的轮状病毒与儿童中流行的病毒株非常相似，尤其是与欧洲和亚洲报告的病毒株具有很高的核苷酸同一性。在儿童和成人中循环相同的轮状病毒株表明，成年人可能通过人群有助于维持轮状病毒的循环。
