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Detection of swine pox and buffalo pox viruses in cell culture using a protein A-horseradish peroxidase conjugate.

作者信息

Mohanty P K, Verma P C, Rai A

机构信息

Division of Biological Products, Indian Veterinary Research Institute, Izatnagar.

出版信息

Acta Virol. 1989 May;33(3):290-6.

PMID:2570516
Abstract

Buffalo pox virus antigen was detected in Vero cells and swine pox virus antigen in the cytoplasm and nucleus of PK-15 and IB-RS-2 cells as early as 6 hr post infection (p.i.) by indirect immunoperoxidase technique using a Protein A-horseradish peroxidase (HRP) conjugate. The viral antigens localized in the cytoplasm of infected cells were the most prominent after 24 hr p.i.

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