Pines J, Hunter T
Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, San Diego, California 92138.
Cell. 1989 Sep 8;58(5):833-46. doi: 10.1016/0092-8674(89)90936-7.
This paper reports the nucleotide and predicted amino acid sequence of a human B-type cyclin. The predicted protein sequence shows strong homology to the other known cyclins in the central third of the protein. We show that the level of cyclin mRNA is regulated during the cell cycle, increasing during G2 phase to four time that present in G1. The protein accumulates steadily during G2 to at least 20 times its level in G1 and is abruptly destroyed at mitosis. In G2/M phase, cyclin is associated with p34cdc2, the human homolog of the fission yeast gene cdc2+, and this complex has histone H1 kinase activity.
本文报道了一种人类B型细胞周期蛋白的核苷酸序列及预测的氨基酸序列。预测的蛋白质序列在蛋白质的中间三分之一区域与其他已知的细胞周期蛋白具有很强的同源性。我们发现细胞周期蛋白mRNA的水平在细胞周期中受到调控，在G2期增加至G1期的四倍。该蛋白质在G2期稳定积累，至少是其在G1期水平的20倍，并在有丝分裂时突然被降解。在G2/M期，细胞周期蛋白与p34cdc2相关联，p34cdc2是裂殖酵母基因cdc2+的人类同源物，并且这种复合物具有组蛋白H1激酶活性。
