Bloemhoff Yris, Forbes Andrew, Good Barbara, Morgan Eric, Mulcahy Grace, Strube Christina, Sayers Ríona
Animal and Bioscience Research Department, Animal & Grassland Research and Innovation Centre, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland.
Merial, 29 Avenue Tony Garnier, 69007 Lyon, France.
Vet Parasitol. 2015 Apr 15;209(1-2):108-16. doi: 10.1016/j.vetpar.2015.01.021. Epub 2015 Feb 4.
Infections with Dictyocaulus viviparus and Ostertagia ostertagi nematode parasites are of importance to bovine health and production in temperate areas across the world. Losses due to these parasites in dairy herds can be considerable due to decreased milk productivity and fertility. However, information on current epidemiological patterns in Irish dairy herds is limited. Bulk milk samples were collected from a total of 319 dairy farms across the Republic of Ireland. The D. viviparus samples were tested with an ELISA based on recombinant major sperm protein, while the O. ostertagi samples were tested with an ELISA based on crude saline extract, whole worm O. ostertagi antigen. Management data were collected from the farms using a questionnaire. Logistic regression was used to find significant associations between the presence of antibodies against D. viviparus and O. ostertagi and management factors. The overall prevalence of D. viviparus infection was 62.8%, while over 98% of herds had antibodies to O. ostertagi at the specified cut-off. Both D. viviparus and O. ostertagi antibodies were highest in November, which could be explained by the accumulated uptake of larvae through the grazing season. In herds of farmers that dosed their in-calf heifers with anthelmintics were significantly more likely to be positive for antibodies against D. viviparus infection. This study highlights that both D. viviparus and O. ostertagi infections are widespread in dairy herds in Ireland throughout the grazing season.
胎生网尾线虫和奥斯特他线虫感染对全球温带地区的牛健康和生产具有重要意义。由于牛奶产量和繁殖力下降，这些寄生虫给奶牛群造成的损失可能相当大。然而，关于爱尔兰奶牛群当前流行病学模式的信息有限。从爱尔兰共和国总共319个奶牛场采集了批量牛奶样本。胎生网尾线虫样本用基于重组主要精子蛋白的酶联免疫吸附测定（ELISA）进行检测，而奥斯特他线虫样本用基于粗盐水提取物、奥斯特他线虫全虫抗原的ELISA进行检测。使用问卷从农场收集管理数据。采用逻辑回归分析来找出抗胎生网尾线虫和奥斯特他线虫抗体的存在与管理因素之间的显著关联。胎生网尾线虫感染的总体患病率为62.8%，而超过98%的牛群在指定临界值时有抗奥斯特他线虫抗体。胎生网尾线虫和奥斯特他线虫抗体在11月最高，这可以通过整个放牧季节幼虫累积摄取来解释。在给怀孕小母牛使用驱虫药的农民的牛群中，抗胎生网尾线虫感染抗体呈阳性的可能性显著更高。这项研究突出表明，在爱尔兰，整个放牧季节胎生网尾线虫和奥斯特他线虫感染在奶牛群中普遍存在。
