Forbes A B, Charlier J
Merial SAS, 29 Avenue Tony Garnier, Lyon 69007, France.
Vet Parasitol. 2008 Oct 20;157(1-2):100-7. doi: 10.1016/j.vetpar.2008.06.023. Epub 2008 Jul 1.
Measurement of antibodies to Ostertagia ostertagi in bulk tank milk (BTM) has value as a diagnostic indicator for potential production losses and anthelmintic treatment responses in dairy herds. Most of the recent data on O. ostertagi antibodies in milk have been generated in Belgium and Canada; the purpose of this study was to determine the range of O. ostertagi antibody levels in several European countries. BTM samples were collected during the autumn of 2005 and 2006 from a total of 1185 dairy herds from dairy farming regions in Denmark, Germany, Ireland, Italy, the Netherlands, Portugal, Spain and the United Kingdom. Antibody titres to O. ostertagi were determined by indirect ELISA and expressed as optical density ratios (ODR). In addition, relationships between ODR and management practices were investigated. For each country the mean ODR and the 25th-75th percentile values were determined. Mean BTM ODR values in herds with access to yards, paddocks and pastures ranged from 0.3 in Italy to 0.6 in Portugal and the UK/Ireland. The BTM ODR values obtained in this study were generally lower than those described in the literature for Belgium, but comparable with those in Canada. Variations between different European countries appeared to reflect different husbandry practices, particularly those related to access to pasture. The association analyses showed correlations between the BTM O. ostertagi ODR, outside access and grazing management, consistent with the publications from Belgium and Canada. When diagnostic values appropriate for different production situations and environments have been further validated, the test will provide an objective, quantitative assessment of the O. ostertagi status of a dairy herd and the possible impact this may have on performance and potential responses to anthelmintic treatment. This represents a significant step forward in evidence-based medicine for dairy veterinarians, advisors and farmers.
测定散装罐奶（BTM）中奥斯特他吉线虫（Ostertagia ostertagi）抗体，对于诊断奶牛群潜在的生产损失及驱虫治疗反应具有重要价值。近期有关牛奶中奥斯特他吉线虫抗体的大部分数据来自比利时和加拿大；本研究的目的是确定几个欧洲国家奥斯特他吉线虫抗体水平的范围。2005年秋季和2006年期间，从丹麦、德国、爱尔兰、意大利、荷兰、葡萄牙、西班牙和英国奶牛养殖地区的总共1185个奶牛群中采集了BTM样本。通过间接ELISA法测定奥斯特他吉线虫的抗体滴度，并以光密度比值（ODR）表示。此外，还研究了ODR与管理措施之间的关系。确定了每个国家的平均ODR以及第25至75百分位数的值。有院子、围场和牧场的牛群中，BTM的平均ODR值范围从意大利的0.3到葡萄牙以及英国/爱尔兰的0.6。本研究获得的BTM ODR值通常低于比利时文献中描述的值，但与加拿大的值相当。不同欧洲国家之间的差异似乎反映了不同的饲养方式，特别是与牧场使用相关的方式。关联分析显示，BTM中奥斯特他吉线虫ODR、外部通道和放牧管理之间存在相关性，这与比利时和加拿大的出版物一致。当针对不同生产情况和环境的诊断值得到进一步验证后，该检测将为奶牛群的奥斯特他吉线虫状况提供客观、定量的评估，以及这可能对生产性能和驱虫治疗潜在反应产生的影响。这对于奶牛兽医、顾问和养殖户而言，是循证医学向前迈出的重要一步。
