Santiago Ronise Martins, Zaminelli Tiago, Bassani Taysa B, Boschen Suelen L, Lima Marcelo M S, Da Cunha Cláudio, Andreatini Roberto, Vital Maria A B F
Department of Pharmacology, Federal University of Paraná, Brazil.
Department of Physiology, Federal University of Paraná, Brazil.
J Pharmacol Pharmacother. 2015 Jan-Mar;6(1):7-12. doi: 10.4103/0976-500X.149133.
To investigate the antidepressant-like effect of piroxicam with a focus on serotonergic neurotransmission.
Rats were randomly distributed into the following groups: 0.9% saline control; 3 mg/kg pizotifen; 10 mg/kg sertraline; 10 mg/kg piroxicam; 10 mg/kg sertraline + 10 mg/kg piroxicam; 10 mg/kg sertraline + 3 mg/kg pizotifen; and 10 mg/kg piroxicam + 3 mg/kg pizotifen. All the drugs were dissolved in 0.9% saline. Three administrations of the drugs (piroxicam and sertraline) were performed 1, 5 and 24 h before testing the animals in the open field followed by the forced swim test (FST). Piroxicam and sertraline were administered orally by gavage and pizotifen was administered intraperitoneally 30 min before gavage. Immediately after the FST, the hippocampi were rapidly dissected for neurochemical analysis in high-performance liquid chromatography.
Acute treatment with piroxicam promoted an antidepressant-like effect in the FST, which was associated with an increase in serotonin levels in the hippocampus. This effect was potentiated in the piroxicam + sertraline group but counteracted by administration of the non-selective serotonin receptor antagonist pizotifen.
These results suggest that the antidepressant-like effect of piroxicam in the FST is mediated by the serotonin system; however, by different mechanisms from those of sertraline.
研究吡罗昔康的抗抑郁样作用，重点关注血清素能神经传递。
将大鼠随机分为以下几组：0.9%生理盐水对照组；3mg/kg苯噻啶；10mg/kg舍曲林；10mg/kg吡罗昔康；10mg/kg舍曲林 + 10mg/kg吡罗昔康；10mg/kg舍曲林 + 3mg/kg苯噻啶；以及10mg/kg吡罗昔康 + 3mg/kg苯噻啶。所有药物均溶于0.9%生理盐水中。在旷场试验中对动物进行测试前1、5和24小时，对药物（吡罗昔康和舍曲林）进行三次给药，随后进行强迫游泳试验（FST）。吡罗昔康和舍曲林通过灌胃口服给药，苯噻啶在灌胃前30分钟腹腔注射。FST结束后，立即迅速解剖海马体，用于高效液相色谱法的神经化学分析。
吡罗昔康急性治疗可促进FST中的抗抑郁样作用，这与海马体中血清素水平的升高有关。在吡罗昔康 + 舍曲林组中这种作用增强，但非选择性血清素受体拮抗剂苯噻啶的给药可抵消这种作用。
这些结果表明，吡罗昔康在FST中的抗抑郁样作用是由血清素系统介导的；然而，其作用机制与舍曲林不同。
