Curran Jerry, Makara Michael A, Mohler Peter J
The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center Columbus, OH, USA ; Department of Physiology and Cell Biology, The Ohio State University Wexner Medical Center Columbus, OH, USA.
The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center Columbus, OH, USA ; Department of Physiology and Cell Biology, The Ohio State University Wexner Medical Center Columbus, OH, USA ; Department of Internal Medicine, The Ohio State University Wexner Medical Center Columbus, OH, USA.
Front Physiol. 2015 Feb 9;6:34. doi: 10.3389/fphys.2015.00034. eCollection 2015.
The ability to dynamically regulate, traffic, retain, and recycle proteins within the cell membrane is fundamental to life and central to the normal function of the heart. In the cardiomyocyte, these pathways are essential for the regulation of Ca(2+), both at the level of the plasma membrane, but also in local cellular domains. One intracellular pathway often overlooked in relation to cardiovascular Ca(2+) regulation and signaling is the endosome-based trafficking pathway. Highlighting its importance, this system and its molecular components are evolutionarily conserved across all metazoans. However, remarkably little is known of how endosome-based protein trafficking and recycling functions within mammalian cells systems, especially in the heart. As the endosomal system acts to regulate the expression and localization of membrane proteins central for cardiac Ca(2+) regulation, understanding the in vivo function of this system in the heart is critical. This review will focus on endosome-based protein trafficking in the heart in both health and disease with special emphasis for the role of endocytic regulatory proteins, C-terminal Eps15 homology domain-containing proteins (EHDs).
在细胞膜内动态调节、运输、保留和循环利用蛋白质的能力是生命的基础，也是心脏正常功能的核心。在心肌细胞中，这些途径对于钙离子（Ca(2+)）的调节至关重要，不仅在质膜水平，而且在局部细胞区域也是如此。在心血管Ca(2+)调节和信号传导方面，一个经常被忽视的细胞内途径是基于内体的运输途径。该系统及其分子成分在所有后生动物中都具有进化保守性，这突出了其重要性。然而，对于基于内体的蛋白质运输和循环在哺乳动物细胞系统中，尤其是在心脏中的功能，人们所知甚少。由于内体系统负责调节对心脏Ca(2+)调节至关重要的膜蛋白的表达和定位，了解该系统在心脏中的体内功能至关重要。本综述将重点关注健康和疾病状态下心脏中基于内体的蛋白质运输，特别强调内吞调节蛋白，即含C末端Eps15同源结构域蛋白（EHDs）的作用。
