*Research Institute, Chronic Viral Illness Service, and Division of Clinical Immunology, McGill University Health Centre, Montreal, Quebec, Canada; Division of Experimental Medicine, McGill University, Montreal, Quebec, Canada; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montreal, Quebec, Canada; and Department of Family Medicine, Université de Montréal, Montreal, Quebec, Canada.
*Research Institute, Chronic Viral Illness Service, and Division of Clinical Immunology, McGill University Health Centre, Montreal, Quebec, Canada; Division of Experimental Medicine, McGill University, Montreal, Quebec, Canada; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montreal, Quebec, Canada; and Department of Family Medicine, Université de Montréal, Montreal, Quebec, Canada
J Leukoc Biol. 2015 Apr;97(4):761-7. doi: 10.1189/jlb.4AB1014-499R. Epub 2015 Feb 20.
HLA-null cell lines [721.221 (henceforth, 721) and K562] are often used to study NK cell activation. NK cells are innate immune lymphocytes that express a variety of stochastically expressed inhibitory and activating receptors. Although it is known that 721 and K562 have divergent origins, they have been used interchangeably to stimulate NK cells in many studies. We hypothesized that the differences between 721 and K562 cells may result in differential NK cell-activation patterns. In this report, we assessed all possible combinations of CD107a expression and IFN-γ and CCL4 secretion in total NK and 3DL1(+/-) NK cell populations induced by these 2 cell lines. 721 activates a significantly higher frequency of NK cells and 3DL1(+) NK cells than K562. The NK cell functional subsets that are stimulated to a higher degree by 721 than K562 include those secreting IFN-γ and/or CCL4. On the other hand, the functional subsets that include CD107 expression contribute to a higher proportion of the total NK cell response following stimulation with K562 than 721. These results have implications for the selection of HLA-null cell lines to use as NK cell stimuli in investigations of their role in infectious diseases, cancer, and transplantation.
HLA-null 细胞系[721.221（简称 721）和 K562]常用于研究 NK 细胞激活。NK 细胞是先天免疫淋巴细胞，表达多种随机表达的抑制性和激活性受体。尽管已知 721 和 K562 具有不同的起源，但在许多研究中，它们被互换用于刺激 NK 细胞。我们假设 721 和 K562 细胞之间的差异可能导致 NK 细胞激活模式的差异。在本报告中，我们评估了这两种细胞系诱导的总 NK 和 3DL1(+/-)NK 细胞群体中 CD107a 表达和 IFN-γ和 CCL4 分泌的所有可能组合。721 比 K562 激活更高频率的 NK 细胞和 3DL1(+)NK 细胞。与 K562 相比，721 更强烈刺激的 NK 细胞功能亚群包括分泌 IFN-γ和/或 CCL4 的亚群。另一方面，与 K562 相比，CD107 表达的功能亚群在刺激后对总 NK 细胞反应的贡献比例更高。这些结果对选择 HLA-null 细胞系作为 NK 细胞刺激物以研究其在传染病、癌症和移植中的作用具有重要意义。
